Chromatography troubleshooting (Continued)
Symptom
Baseline noise,
long-term
(approximately 1 hour)
cycling
Straight baseline, no
peaks
3-20
Troubleshooting, Testing, and Maintenance
Possible cause
Ambient temperature
fluctuations
Integrator or recorder
faulty
No pump flow
Lamp not on
Detector not zeroed
Improper connection
between Prep system
and recorder
Incorrect wavelength
Leak in solvent path
Corrective action
Stabilize operating
environment
temperature.
Check integrator or
recorder for excessive
baseline noise.
Set pump flow rate.
Troubleshoot detector.
Zero detector baseline.
Check cabling between
Prep system unit and
recorder.
Check wavelength
setting.
Check fittings.