Waters Prep Series Installation And Maintenance Manual page 95

Erratic retention time troubleshooting
Retention time
problem
No
Retention
time erratic?
Yes
Are pressure
Yes
fluctuations same
as during normal
operation?
No
Prime pump
to remove
air bubbles
Problem
corrected?
No
Yes
System
leaking?
No
Troubleshoot pump:
- Seals
- Check valves
- Plunger
No
Call Waters
technical service
Ret time
incorrect, but
reproducible?
Yes
Chemistry
problem?
No
Problem Is
operation
Yes
Wrong
column
Equilibrate
equilibration? column
No
Yes
Temp
Stabilize
fluctuations
environment
No
Suggested actions:
Yes
Column -Troubleshoot
column
contaminated?
- Improve sample
prep
Isolate and
troubleshoot
leak
See next page
Yes
Mobile phase
degrading? mobile phase
No
Yes
Mobile phase
separating?
No
No
Suggested actions:
Sparge conditions
causing solvent
- Decrease sparge
Yes
components to
evaporate at
different rates
Troubleshooting
Use fresh
Stir mobile
phase
rate
- Use separate
reservoir for pure
solvents
- Allow pump to
make mixture.
Mixing does not
apply to RI
detectors.
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