Waters Prep Series Installation And Maintenance Manual page 128

2. Fill reservoirs C and D with solvent 2 (100% methanol with UV
absorbing sample added).
The concentration of UV absorbing sample used in solvent 2 should produce
about 0.45 AUFS in this test.
Flushing solvent lines
Be sure to flush any residual solvents from the system.
Adjusting the recorder
To adjust the recorder:
1. Set the detector to 0.5 AUFS.
2. Pump solvent 1 (reservoir A or B) isocratically at 5 mL/min and adjust
the recorder
to 0.
3. Pump solvent 2 (reservoir C or D) isocratically at 5 mL/min, and adjust
the recorder to full scale using the recorder's variable full scale input.
The recorder is now set to respond so that 0% composition (pure methanol in
solvent 1) = 0% full scale, and 100% composition (methanol containing sample
in solvent 2) = 100% full scale.
Program the gradient table
Enter and save Gradient table values as shown below.
Gradient table values
Time Flow
Initial 1.00
5.00 1.00
10.00 1.00
15.00 1.00
20.00 1.00
25.00 1.00
30.00 1.00
35.00 1.00
40.00 1.00
3-44 Troubleshooting, Testing, and Maintenance
%A %B
100 0
90 0
100 0
90 0
0 100
0 90
0 100
0 90
0 100
%C %D
0 0
10 0
0 0
0 10
0 0
10 0
0 0
0 10
0 0
Curve
*
11
11
11
11
11
11
11
11