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Waters ACQUITY CM-30S Overview And Maintenance Manual

30-cm single zone column manager
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ACQUITY 30-cm Single-
Zone Column Manager
Overview and Maintenance Guide
715004178 / Revision B
Copyright © Waters Corporation 2014
All rights reserved

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Summary of Contents for Waters ACQUITY CM-30S

  • Page 1 ACQUITY 30-cm Single- Zone Column Manager Overview and Maintenance Guide 715004178 / Revision B Copyright © Waters Corporation 2014 All rights reserved...
  • Page 2 October 7, 2014, 715004178 Rev. B...

  • Page 3: Copyright Notice

    Corporation assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, its use. For the most recent revision of this document, consult the Waters Web site (waters.com).

  • Page 4: Contacting Waters

    Contacting Waters ® Contact Waters with enhancement requests or technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information: Contacting medium Information...

  • Page 5: Considerations Specific To The 30-Cm Single-Zone Column Manager

    Warning: United States and HAR-type (or better) in Europe. The main power cord must only be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. High voltage hazard...

  • Page 6: Electrical Power Safety Notice

    Electrical power safety notice Do not position the instrument so that it is difficult to operate the disconnecting device. Equipment misuse notice If the equipment is used in a manner not specified by the manufacturer, the protection provided by the equipment may be impaired. Safety advisories Consult Appendix A...

  • Page 7: Applicable Symbols

    For compliance with the Waste Electrical and Electronic Equipment Directive (WEEE) 2012/19/EU, contact Waters Corporation for the correct disposal and recycling instructions. Serial number October 7, 2014, 715004178 Rev. B...

  • Page 8: Audience And Purpose

    30-cm Single-Zone Column Manager (CM-30S). It gives an overview of the technology and operation of the CM-30S. Intended use of the 30-cm single-zone column manager Waters designed the 30-cm, single zone column manager for use in Advanced ™ ™ Polymer Chromatography...

  • Page 9: Emc Considerations

    Class B products are suitable for use in both commercial and residential locations and can be directly connected to a low voltage, power-supply network. EC authorized representative Waters Corporation Stamford Avenue Altrincham Road Wilmslow SK9 4AX UK Telephone:...
  • Page 10 October 7, 2014, 715004178 Rev. B...

  • Page 11: Table Of Contents

    General Information ..................iii Copyright notice ....................iii Trademarks ......................iii Customer comments .................... iii Contacting Waters ....................iv Safety considerations ..................iv Safety hazard symbol notice................iv Considerations specific to the 30-cm single-zone column manager ....v FCC radiation emissions notice ................v Electrical power safety notice ................
  • Page 12 2 Preparing and operating the CM-30S ..........23 Securing the CM-30S to the system stack ............ 24 Installing the leak sensor ................. 32 Installing the waste tubing ................34 Connecting to the electricity source ............. 38 Monitoring LEDs ....................38 CM-30S control panel ..................
  • Page 13 A Safety Advisories .................. 127 Warning symbols ....................127 Specific warnings ..................... 129 Notices ........................ 130 Warnings that apply to all Waters instruments and devices ....131 Electrical and handling symbols ..............136 Electrical symbols .................... 136 Handling symbols .................... 137 B Specifications ..................
  • Page 14 October 7, 2014, 715004178 Rev. B...

  • Page 15: 30-Cm Single-Zone Column Manager

    30-cm single-zone column manager Variations in column temperature can shift peak retention times, altering peak shapes and increasing the difficulty of achieving precise results. The 30-cm single-zone column manager (CM-30S) helps ensure precise, reproducible separations by controlling the column temperature. Contents: Topic Page...

  • Page 16: Location Within The System

    1 30-cm single-zone column manager ACQUITY UPC and GPC columns are equipped with eCord Information Management Technology, which captures the history of each column, to assist in tracking column usage. Reusable high-pressure fittings facilitate the replacement of the columns. The CM-30S can accommodate as many as eight, 30-cm columns with inside diameters of 2.1 mm to 8.0 mm.
  • Page 17 Location within the system Location of the CM-30S within a typical APC system: Solvent-bottle tray Detector Single-zone 30-cm, single-zone column manager column manager Sample manager Solvent manager Risk of tipping. To avoid injury, ensure the side-mount shaft Warning: on the right-hand side of the sample manager or single-zone column manager (CM-S) is engaged in the key-hole slot on the mounting bracket on the left-hand side of the CM-30S.

  • Page 18: Major Components

    1 30-cm single-zone column manager Major components The following diagrams show the CM-30S’s major components. CM-30S, with drawer of column compartment closed: Inlet valve (for Outlet valve (for UPC only) only) Valve drip tray iButtons (eCord receptacles) - 1 through 4 for UPC - 1a through 1d for APC Drawer handle iButtons (eCord receptacles)
  • Page 19 Major components CM-30S components: Component Description Drawer handle Disengages the column compartment’s door latch, to allow access to the column plate. Drain fitting (to Routes waste from the leak management system flow waste) through this connector to the waste container. Drip tray Manages fluid leaks.
  • Page 20 1 30-cm single-zone column manager CM-30S column compartment (left-hand side shown): Inlet and outlet valves (for UPC only) Passive preheater assembly - 8 for UPC (4 on left-hand side and 4 on right-hand side) - 1 to 2 for APC (1 on left-hand side and 1 optional on right-hand side) Column plate latch...
  • Page 21 Major components CM-30S components: (Continued) Component Description Column plate When disengaged, allows removal of the column plate. latch Column drawer A nonremovable drawer in the column compartment that allows access to the column plates and passive preheaters. Column-outlet In UPC systems, the column-outlet tubing connects the tubing column outlet to the CM-30S outlet valve.
  • Page 22 1 30-cm single-zone column manager CM-30S rear panel: RS-232 cable connection port Ethernet cable connection port Power cord receptacle October 7, 2014, 715004178 Rev. B...

  • Page 23: Preparing And Operating The Cm-30S

    Preparing and operating the CM-30S Contents: Topic Page Securing the CM-30S to the system stack ........24 Installing the leak sensor ..............32 Installing the waste tubing ............. 34 Connecting to the electricity source ..........38 Monitoring LEDs................38 CM-30S control panel ..............39 Installation recommendations for fittings ........

  • Page 24: Securing The Cm-30S To The System Stack

    2 Preparing and operating the CM-30S To avoid the harmful effects of personal contact with solvents, Warning: including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use. Risk of tipping. To avoid injury, ensure the side-mount shaft Warning: on the right-hand side of the sample manager or single-zone column manager (CM-S) is engaged in the key-hole slot on the mounting bracket...
  • Page 25 Securing the CM-30S to the system stack Using the T20 TORX screwdriver, remove the upper-left screw from the right side panel of the sample manager or CM-S, and retain the screw. Upper-left screw Position the mounting bracket over the two upper right side panel screw holes, and then use the T20 TORX screwdriver to secure the left side of the bracket with the M4 ×...
  • Page 26 2 Preparing and operating the CM-30S Using the T20 TORX screwdriver, secure the right side of the mounting bracket with the screw you removed in step Screw Mounting bracket October 7, 2014, 715004178 Rev. B...
  • Page 27 Securing the CM-30S to the system stack Insert the M4 × 50-mm pan head screw into the collar flange, and then use the T20 TORX driver to mount the collar on the left side of the mounting bracket on the sample manager or CM-S. Left side of bracket Collar flange M4 ×...
  • Page 28 2 Preparing and operating the CM-30S Carefully slide the CM-30S toward the sample manager or CM-S so that the L-shaped support bar at the bottom, rear of the CM-30S is inserted under the base of the BSM. Rear of L-shaped Base of BSM CM-30S support bar...
  • Page 29 Securing the CM-30S to the system stack Loosen the upper and lower thumbscrews on the CM-30S mounting bracket, slide the bracket upward, and then position it so that the two notched areas are resting on both thumbscrews. Upper thumbscrew Notched area Lower thumbscrew Notched area October 7, 2014, 715004178 Rev.
  • Page 30 2 Preparing and operating the CM-30S Insert the sample manager or CM-S collar’s flange into the wide portion of the upper cutout on the CM-30S mounting bracket. Thumbscrew in notched area Sample manager or CM-S collar’s flange Wide portion of upper cutout on CM-30S mounting bracket Thumbscrew in notched area October 7, 2014, 715004178 Rev.
  • Page 31 Securing the CM-30S to the system stack 10. Slide the CM-30S mounting bracket fully down, and then re-tighten the two thumbscrews. Upper thumbscrew CM-30S mounting bracket Lower thumbscrew 11. Loosen the collar to secure the CM-30S to the sample manager or CM-S. Collar October 7, 2014, 715004178 Rev.

  • Page 32: Installing The Leak Sensor

    2 Preparing and operating the CM-30S Installing the leak sensor To avoid the harmful effects of personal contact with solvents, Warning: including inhalation, observe Good Laboratory Practice when you handle them. See the Material Safety Data Sheets for the solvents you use.
  • Page 33 Installing the leak sensor Open the outer door of the CM-30S. Outer door handle Carefully unpack the new leak sensor. Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place. T-bar Slot in leak sensor reservoir...

  • Page 34: Installing The Waste Tubing

    For additional information on configuring waste tubing, see the See also: Waters ACQUITY Advanced Polymer Chromatography (APC) System Site Preparation Guide or the ACQUITY UPC Site Preparation Guide. Required materials •...
  • Page 35 Installing the waste tubing To install and route the waste tubing: Wet the barbed drain fitting located at the bottom of the CM-30S using methanol. Slide the waste tubing over the barbed drain fitting, and route the tubing to a suitable waste container. Barbed drain fitting October 7, 2014, 715004178 Rev.
  • Page 36 2 Preparing and operating the CM-30S To avoid releasing solvent vapors into the room, route Warning: vent tubing from the waste container to a fume hood or other, suitable exhaust system. To avoid spills, empty the waste container at regular Warning: intervals.
  • Page 37 Installing the waste tubing To avoid pressure buildup in the vent tubing of the waste Notice: container, ensure the tubing does not crimp or bend sharply. The tubing must freely vent gas and vapor to a fume hood or laboratory exhaust system.

  • Page 38: Connecting To The Electricity Source

    • Use power cord SVT-type in the United States and HAR-type or better in Europe. For other countries’ requirements, contact your local Waters distributor. • Inspect the power cord for damage and replace it, if necessary. • Power-off and unplug the CM-30S before performing any maintenance operation on it.

  • Page 39: Cm-30S Control Panel

    CM-30S control panel Run LED indications: LED mode and color Description Unlit If both run and power LED are unlit, the CM-30S is powered off. If run LED is unlit and power LED is constant green, the CM-30S is in idle mode. Blinking green The CM-30S is initializing or preparing to run a method.
  • Page 40 2 Preparing and operating the CM-30S When monitoring plots in the instrument console, the “Waste” valve Note: position, when selected, will appear as “-1”, the “Bypass” valve position will appear as “-2”, and in multi-column manager systems, “None” will appear as “-5”.

  • Page 41: Installation Recommendations For Fittings

    Installation recommendations for fittings You can access additional functions by right-clicking anywhere in the CM-30S control panel. Additional functions in the CM-30S control panel: Control panel function Description Launch instrument console Launches the instrument console. Reset CM Resets the CM-30S after an error condition.
  • Page 42 2 Preparing and operating the CM-30S When tightening system fittings, consult the following table. Installation recommendations for ACQUITY APC and UPC column-compartment fittings: Fitting Recommended tightening Stainless steel (gold-plated) fitting with 1. Using your fingers, screw long flats and 2-piece stainless steel the gold fitting into the ferrule on hypo-extended tubing port and stop as soon as...

  • Page 43: Assembling New Fittings

    Assembling new fittings Installation recommendations for ACQUITY APC and UPC column-compartment fittings: (Continued) Fitting Recommended tightening Stainless steel (gold-plated) fitting with Finger-tight, plus 3/4-turn long flats and 2-piece stainless steel using wrench ferrule 3/4-turn TP03378 Long flats 2-piece ferrule Stainless steel (gold-plated) fitting with Finger-tight, plus up to long flats and 2-piece stainless steel 1/6-turn using wrench...

  • Page 44: Installing Dual-Threaded, Finger-Tight Fittings

    2 Preparing and operating the CM-30S To assemble new fittings: Insert the end of a tube into the hexagonal end of the compression screw. Insert the tube into the larger end of the ferrule. Insert the tube into the fitting body. Rotate the compression screw, clockwise, into the fitting body until the screw is finger-tight.

  • Page 45: Installing Or Replacing A Column Plate In The Column Compartment

    Installing or replacing a column plate in the column compartment Insert the stainless-steel tubing, with ferrule, into the column. Seat the ferrule by finger tightening the dual-threaded fitting. Do not rotate the stainless-steel silver cap nut when Requirement: seating the ferrule. Complete the connection by finger tightening the cap nut onto the dual-threaded fitting.
  • Page 46 2 Preparing and operating the CM-30S To install a column plate: Open the outer door of the CM-30S. Outer door handle Lift the handle of the column-compartment drawer up, to release the door latch. Drawer handle October 7, 2014, 715004178 Rev. B...
  • Page 47 Installing or replacing a column plate in the column compartment Grasp the handle of the column-compartment drawer, and pull the drawer toward you. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...
  • Page 48 2 Preparing and operating the CM-30S If a column plate was previously installed, ensure that the column fittings are disconnected from the column, release the column plate by pushing up on the latch or pushing down on the lever, and then remove the column plate from the column compartment.
  • Page 49 Installing or replacing a column plate in the column compartment After the plate bottoms on the lower guide, push the plate toward the latch until the latch engages the plate. Latch Column plate Slide the column upward toward the fitting, and insert the passive preheater tubing, with the fitting and ferrule, into the inlet end of the column or column bank.
  • Page 50 2 Preparing and operating the CM-30S Tighten the fitting, referring to the instructions for the “Dual-threaded, finger-tight fitting with cap nut” on page Fitting Column Insert the outlet tubing, with the fitting and ferrule, into the outlet end of the column or column bank. Outlet end of column Outlet tubing with fitting and ferrule October 7, 2014, 715004178 Rev.
  • Page 51 Installing or replacing a column plate in the column compartment 10. Tighten the fitting referring to the instructions for the “Dual-threaded, finger-tight fitting with cap nut” on page Column Fitting 11. If you are installing the column plate on the left-hand side of the compartment, thread the eCords through the eCord guide on the back of the column rack.
  • Page 52 2 Preparing and operating the CM-30S 12. To prevent loose tubing from interfering with the operation of the column-compartment drawer and outer CM-30S door, route the column-outlet tubing through a column clip on the column plate. Column plate Column-outlet tubing Column clip 13.
  • Page 53 Installing or replacing a column plate in the column compartment 14. Grasp the handle of the column-compartment drawer, and push the drawer in. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...

  • Page 54: Installing And Removing A Single Column On The Column Plate

    2 Preparing and operating the CM-30S 15. Ensure the tubing or eCord fob cable does not interfere with the latch mechanism, and then close the column-compartment drawer while pushing down on the handle, to secure the latch. Drawer handle 16. Close the outer door of the CM-30S. Installing and removing a single column on the column plate Required materials...
  • Page 55 Installing and removing a single column on the column plate Insert at least two column support clips through the large, square hole on the bottom of the column plate. Column support clip Column plate Large, square hole Press the column support clips into the column plate and slide the clips along the track until they are in the desired position on the column plate.
  • Page 56 2 Preparing and operating the CM-30S Open each column support clip by squeezing its sides and then swinging the spring clip outward. Spring clip Side Side Column support clip Snap the column into each column support clip. Column Column support clip October 7, 2014, 715004178 Rev.
  • Page 57 Installing and removing a single column on the column plate Close each spring clip, to lock the column in place. Spring clip Column To remove a single column from the column plate: Open each column support clip by squeezing its sides and then swinging the spring clip outward.

  • Page 58: Examples Of Correct Column Support Clip Placement

    2 Preparing and operating the CM-30S Remove the column from the column support clips. Column Column support clip Examples of correct column support clip placement The following diagrams show the recommended placement of column support clips for columns of varying length. Column support clips are not required for all column configurations.
  • Page 59 Installing and removing a single column on the column plate 300-mm columns with correct clips in recommended positions: Column support clip 300-mm column 300-mm column with filter 300-mm column with 30-mm guard column 300-mm column with 50-mm guard column Column plate October 7, 2014, 715004178 Rev.
  • Page 60 2 Preparing and operating the CM-30S 250-mm columns with correct clips in recommended locations: Column support clip 250-mm column 250-mm column with filter 250-mm column with 30-mm guard column 250-mm column with 50-mm guard column Column plate October 7, 2014, 715004178 Rev. B...
  • Page 61 Installing and removing a single column on the column plate 150-mm columns with correct clips in recommended locations: Column support clip 150-mm column 150-mm column with filter 150-mm column with 30-mm guard column 150-mm column with 50-mm guard column Column plate October 7, 2014, 715004178 Rev.
  • Page 62 2 Preparing and operating the CM-30S 100-mm columns with correct clips in recommended locations: Column support clip 100-mm column 100-mm column with filter 100-mm column with 30-mm 100-mm column guard column with 50-mm guard column Column plate October 7, 2014, 715004178 Rev. B...
  • Page 63 Installing and removing a single column on the column plate 75-mm columns with correct clips in recommended locations: Column support clip 75-mm column 75-mm column with filter 75-mm column with 30-mm guard column 75-mm column with 50-mm guard column Column plate October 7, 2014, 715004178 Rev.
  • Page 64 2 Preparing and operating the CM-30S 50-mm columns with correct clips in recommended locations: 50-mm column Column support clip 50-mm column with filter 50-mm column 50-mm column with 30-mm with 50-mm guard column guard column Column plate October 7, 2014, 715004178 Rev. B...

  • Page 65: Connecting An External Valve

    Connecting an external valve Connecting an external valve The CM-30S supports up to three external valves. Each external valve must have a unique address (0, 1, or 2). Required materials • 3-gang, RS-232 serial-adaptor cable • Female-to-female serial cable coupler adapter (up to 3, depending on the number of external valves) •...
  • Page 66 2 Preparing and operating the CM-30S Connect the external valve’s serial cable to a female-to-female serial cable coupler adapter, and then connect the coupler adapter to the connector labeled “1” on the 3-gang RS-232 serial-adaptor cable. Female-to-female Connector on 3-gang serial cable coupler External valve’s RS-232 serial-adaptor...

  • Page 67: Maintaining The 30-Cm Single-Zone Column Manager

    Maintaining the 30-cm single-zone column manager Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. Contents: Topic Page Maintenance schedule ..............67 Spare parts ..................68 Safety and handling ................. 68 Resolving leak sensor errors............69 Replacing the leak sensor ..............

  • Page 68: Spare Parts

    As needed page 125 lint-free cloth, or paper dampened with water Spare parts To ensure that your system operates as designed, use only Waters Quality ® Parts . Visit www.waters.com/wqp for information about Waters Quality Parts, including how to order them.

  • Page 69: Resolving Leak Sensor Errors

    Resolving leak sensor errors To avoid electric shock, do not remove the instrument’s panels. Warning: There are no user-serviceable items inside the instrument. Risk of tipping. To avoid injury, ensure the side-mount shaft Warning: on the right-hand side of the sample manager is engaged in the key-hole slot on the mounting bracket on the left-hand side of the CM-30S.
  • Page 70 3 Maintaining the 30-cm single-zone column manager To restore the leak sensor to service: To avoid damaging the electronic components or circuitry Notice: of a system module, do not disconnect an electrical assembly from the module while the module remains connected to the ac supply source.
  • Page 71 Resolving leak sensor errors Detach the leak sensor connector from the front of the CM-30S by pressing down on its tab and pulling the connector out of the receptacle. Press down on tab to release connector To avoid damaging the leak sensor, do not grasp it by the Notice: ribbon cable.
  • Page 72 3 Maintaining the 30-cm single-zone column manager Roll a nonabrasive, lint-free wipe, and use it to absorb the liquid from the leak sensor reservoir and its surrounding area. With a cotton swab, absorb any remaining liquid from the corners of the leak sensor reservoir and its surrounding area.

  • Page 73: Replacing The Leak Sensor

    Replacing the leak sensor 12. In the column manager information window, click Control > Reset CM, to reset the CM-30S. Replacing the leak sensor Perform this procedure if you must replace the leak sensor in the CM-30S. To avoid personal contamination from contact with Warning: biologically hazardous or toxic materials, always wear clean, chemical-resistant, powder-free gloves when performing this...
  • Page 74 3 Maintaining the 30-cm single-zone column manager Open the outer door of the CM-30S. Outer door handle Press down on the tab, to detach the leak sensor connector from the front of the CM-30S. Press down on tab to release connector October 7, 2014, 715004178 Rev.
  • Page 75 Replacing the leak sensor To avoid damaging the leak sensor, do not grasp it by the Notice: ribbon cable. Remove the leak sensor from its reservoir, grasping it by its serrations, and pulling upward on it. Leak sensor: Serrations Unpack the new leak sensor. Align the leak sensor’s T-bar with the slot in the side of the leak sensor reservoir, and slide the leak sensor into place.

  • Page 76: Replacing The Column-Outlet Tubing In A Cm-30S Installed In An Apc System

    3 Maintaining the 30-cm single-zone column manager Plug the leak sensor connector into the receptacle on the front of the CM-30S. Leak sensor connector receptacle Power-on the CM-30S. In the instrument console, select Column Manager from the system tree. 10. In the column manager information window, click Control > Reset CM, to reset the CM-30S.
  • Page 77 Replacing the column-outlet tubing in a CM-30S installed in an APC system • 1/4-inch open-end wrench • T20 TORX driver Illustrations in the following procedure depict changing column-outlet Note: tubing on the left-hand side of the column compartment. The illustrations also apply to changing a column-outlet tubing on the right-hand side of the column compartment.
  • Page 78 3 Maintaining the 30-cm single-zone column manager Lift the drawer handle of the column compartment to release the door latch. Drawer handle October 7, 2014, 715004178 Rev. B...
  • Page 79 Replacing the column-outlet tubing in a CM-30S installed in an APC system Grasp the handle of the column-compartment drawer, and pull the drawer toward you. Drawer handle Column-compartment drawer Use the 1/4-inch open-end wrench to disconnect the column outlet fitting from the primary column manager outlet valve.
  • Page 80 3 Maintaining the 30-cm single-zone column manager If a column is installed on the column-outlet tubing that you are replacing, do as follows: Loosen the cap nut from the column fitting, taking care not to remove the cap nut. Column Column fitting Cap nut Unscrew the column fitting from the column, and then disconnect...
  • Page 81 Replacing the column-outlet tubing in a CM-30S installed in an APC system Unclip the tubing guide that is on the same side as the column tubing that you are replacing, and remove the tubing. Tubing Tubing guide unclipped To install the new column-outlet tubing: If you are installing a column on the column-outlet tubing that you are replacing, do as follows: Ensure the cap nut is loose on the column fitting, insert the...
  • Page 82 3 Maintaining the 30-cm single-zone column manager Tighten the cap nut onto the column fitting. Column Column fitting Cap nut Column-outlet tubing Insert the tubing into the tubing guide that is on the same side as the column tubing that, and clip the tubing guide closed. Tubing guide clipped Tubing...
  • Page 83 Replacing the column-outlet tubing in a CM-30S installed in an APC system Grasp the handle of the column-compartment drawer, and push the drawer in. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...

  • Page 84: Replacing The Column-Outlet Tubing In A Cm-30S Installed In A Upc 2 System

    3 Maintaining the 30-cm single-zone column manager Close the column-compartment drawer, and push downward on the handle, to secure the latch. Drawer handle Close the outer door of the CM-30S. Replacing the column-outlet tubing in a CM-30S installed in a UPC system To prevent burn injuries, set the column temperature to Off, Warning:...
  • Page 85 Replacing the column-outlet tubing in a CM-30S installed in a UPC system • 1/4-inch open-end wrench • T20 TORX driver Illustrations in the following procedure depict changing column-outlet Note: tubing on the left-hand side of the column compartment. Yet the illustrations equally apply to changing a column-outlet tubing on the right-hand side of the column compartment.
  • Page 86 3 Maintaining the 30-cm single-zone column manager Lift the drawer handle of the column compartment to release the door latch. Drawer handle October 7, 2014, 715004178 Rev. B...
  • Page 87 Replacing the column-outlet tubing in a CM-30S installed in a UPC system Grasp the handle of the column-compartment drawer, and pull the drawer toward you. Drawer handle Column-compartment drawer Using the T20 TORX driver, loosen the two captive screws that secure the tubing-retainer plate, and then carefully remove the plate.
  • Page 88 3 Maintaining the 30-cm single-zone column manager Note the number associated with the position of the column-outlet tubing that you are replacing, and then use the 1/4-inch open-end wrench to disconnect the column outlet fitting from the outlet valve of the CM-30S.
  • Page 89 Replacing the column-outlet tubing in a CM-30S installed in a UPC system If no column is installed on the column-outlet tubing that you are replacing, remove the tubing from the column clip. Column clip Column plate Column-outlet tubing If a column is installed on the column-outlet tubing that you are replacing, do as follows: Loosen the cap nut from the column fitting, taking care not to remove the cap nut.
  • Page 90 3 Maintaining the 30-cm single-zone column manager Unscrew the column fitting from the column, and then disconnect the column-outlet tubing from the column. Column Column fitting Cap nut 10. Depending on the position of the column tubing that you are removing, do one of these things: If you are removing the column tubing from position 1, 2, 3, or 4, unclip the tubing guides on the left and right-hand sides of the...
  • Page 91 Replacing the column-outlet tubing in a CM-30S installed in a UPC system To install the new column-outlet tubing: To avoid spills, do not remove the valves' drip tray when Warning: removing the column-outlet tubing. All drip trays must remain in place during the CM-30S's operation and maintenance.
  • Page 92 3 Maintaining the 30-cm single-zone column manager Bend the tubing so that it immediately extends upward after the fitting, and then back towards the rear of the drip tray, as shown in the figure below. The bending ensures that this section of tubing is the lowest Note: portion exiting from the valve so that any drips fall into the drip tray.
  • Page 93 Replacing the column-outlet tubing in a CM-30S installed in a UPC system If you are not installing a column on the column-outlet tubing that you are replacing, route the tubing through a column clip on the column plate. Doing so prevents loose tubing from interfering with the Rationale: operation of the column-compartment drawer and outer CM-30S door.
  • Page 94 3 Maintaining the 30-cm single-zone column manager Tighten the cap nut onto the column fitting. Column Column fitting Cap nut Column-outlet tubing Depending on the position of the column tubing that you are replacing, do one of these things: If you are replacing the column tubing from position 1, 2, 3, or 4, insert the tubing into the tubing guides on the left and right-hand sides of the column compartment, and clip the tubing guides closed.
  • Page 95 Replacing the column-outlet tubing in a CM-30S installed in a UPC system column compartment, and clip the tubing guides closed. To avoid damaging the tubing, ensure that all tubing lies Notice: flat before reinstalling the tubing-retainer plate. Using the T20 TORX driver, reinstall the tubing-retainer plate, ensuring that all the tubing is contained behind the plate.
  • Page 96 3 Maintaining the 30-cm single-zone column manager Grasp the handle of the column-compartment drawer, and push the drawer in. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...

  • Page 97: Replacing The Passive-Preheater Assembly In A Cm-30S Installed In An Apc System

    Replacing the passive-preheater assembly in a CM-30S installed in an APC system Close the column-compartment drawer, and push downward on the handle, to secure the latch. Drawer handle 10. Close the outer door of the CM-30S. Replacing the passive-preheater assembly in a CM-30S installed in an APC system To prevent burn injuries, set the column temperature to Off, Warning:...
  • Page 98 3 Maintaining the 30-cm single-zone column manager • T20 TORX driver • Torque driver To remove the passive-preheater assembly: Stop any running mobile phase before replacing the passive-preheater assembly. After allowing sufficient time for cooling, open the outer door of the CM-30S.
  • Page 99 Replacing the passive-preheater assembly in a CM-30S installed in an APC system Lift the handle of the column-compartment drawer to release the door latch. Drawer handle October 7, 2014, 715004178 Rev. B...
  • Page 100 3 Maintaining the 30-cm single-zone column manager Grasp the handle of the column-compartment drawer, and pull the drawer toward you. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...
  • Page 101 Replacing the passive-preheater assembly in a CM-30S installed in an APC system Note the number of the passive preheater that you are replacing, and then use the 1/4-inch open-end wrench to disconnect the passive preheater fitting from the inlet valve of the CM-30S. Column positions for the left-hand passive preheater: Triple blank heat sink Position 1a...
  • Page 102 3 Maintaining the 30-cm single-zone column manager To install the new passive-preheater assembly: Install the new passive preheater, and use the T20 TORX driver to secure it in place with the two screws. Screws Use the torque driver to tighten each screw to 16 inch-pounds of torque. Connect the new, passive-preheater tubing to the appropriate position on the primary column manager’s inlet valve, and then tighten the fitting, finger-tight plus an additional 3/4-turn, using the 1/4-inch...
  • Page 103 Replacing the passive-preheater assembly in a CM-30S installed in an APC system Grasp the handle of the column-compartment drawer, and push the drawer in. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...

  • Page 104: Replacing The Passive-Preheater Assembly In A Cm-30S Installed In A Upc System

    3 Maintaining the 30-cm single-zone column manager Close the column-compartment drawer, and push downward on the handle, to secure the latch. Drawer handle Close the outer door of the CM-30S. Replacing the passive-preheater assembly in a CM-30S installed in a UPC system To prevent burn injuries, set the column temperature to Off, Warning:...
  • Page 105 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system A left-hand and right-hand passive preheater assembly is Note: available for ordering. These assemblies contain the required tubing bends for installing the passive preheater in the desired location. •...
  • Page 106 3 Maintaining the 30-cm single-zone column manager Lift the handle of the column-compartment drawer to release the door latch. Drawer handle October 7, 2014, 715004178 Rev. B...
  • Page 107 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system Grasp the handle of the column-compartment drawer, and pull the drawer toward you. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...
  • Page 108 3 Maintaining the 30-cm single-zone column manager Note the number of the passive preheater that you are replacing, and then use the 1/4-inch open-end wrench to disconnect the passive preheater fitting from the inlet valve of the CM-30S. Reference the magnetic diagram on the inside of the CM-30S front Note: door for correct port and column positions.
  • Page 109 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system Column inlet valve: Column inlet valve To avoid spills, do not remove the valves' drip tray when Warning: removing the passive-preheater tubing. All drip trays must remain in place during the CM-30S's operation and maintenance. Using the T20 TORX driver, loosen the two captive screws that secure the tubing-retainer plate, and then carefully remove the plate.
  • Page 110 3 Maintaining the 30-cm single-zone column manager If you are replacing the passive preheater in position 1, 2, 3, or 4, remove its tubing from the upper and lower tubing guide posts adjacent to the left-hand column plate. Upper tubing guide post Lower tubing guide post October 7, 2014, 715004178 Rev.
  • Page 111 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system If you are replacing the passive preheater in position 5, 6, or 7, remove its tubing from the upper and lower tubing guide posts adjacent to the right-hand column plate. Upper tubing guide post Lower tubing guide post If you are replacing the passive preheater in position 8, remove its...
  • Page 112 3 Maintaining the 30-cm single-zone column manager 10. Using the T20 TORX driver, remove the two screws that secure the passive preheater in place, and then remove the preheater. Screws To install the new passive-preheater assembly: Install the new passive preheater, and use the T20 TORX driver to secure it in place with the two screws.
  • Page 113 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system When replacing the passive preheater in position 1, 2, 3, Requirement: or 4, use the left passive preheater assembly. Upper tubing guide post Lower tubing guide post October 7, 2014, 715004178 Rev. B...
  • Page 114 3 Maintaining the 30-cm single-zone column manager If you are replacing the passive preheater in position 5, 6, or 7, route its tubing above the upper tubing guide post and then below the lower tubing guide post adjacent to the right-hand column plate. When replacing the passive preheater in position 5, 6, 7, Requirement: or 8, use the right passive preheater assembly.
  • Page 115 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system If you are replacing the passive preheater in position 8, route its tubing above the upper tubing guide post and then below the middle tubing guide post adjacent to the left-hand column plate. When replacing the passive preheater in position 5, 6, 7, Requirement: or 8, use the right passive preheater assembly.
  • Page 116 3 Maintaining the 30-cm single-zone column manager Bend the tubing so that it immediately extends upward after the fitting, and then back towards the rear of the drip tray, as shown in the figure after step The bending ensures that this section of tubing is the lowest Note: portion exiting from the valve so that any drips fall into the drip tray.
  • Page 117 Replacing the passive-preheater assembly in a CM-30S installed in a UPC system To avoid damaging the tubing, ensure that all tubing lies Notice: flat before reinstalling the tubing-retainer plate. 10. Using the T20 TORX driver, reinstall the tubing-retainer plate, ensuring that all the tubing is contained behind the plate. Tubing Tubing-retainer plate Captive screws...
  • Page 118 3 Maintaining the 30-cm single-zone column manager 11. Grasp the handle of the column-compartment drawer, and push the drawer in. Drawer handle Column-compartment drawer October 7, 2014, 715004178 Rev. B...

  • Page 119: Replacing The Ferrule On The Column Fitting

    Replacing the ferrule on the column fitting 12. Close the column-compartment drawer, and push downward on the handle, to secure the latch. Drawer handle 13. Close the outer door of the CM-30S. Replacing the ferrule on the column fitting Replace the ferrule on the column fitting if the column is leaking. To prevent burn injuries, set the column temperature to Off, Warning: and then allow the column compartment and its components to cool for...
  • Page 120 3 Maintaining the 30-cm single-zone column manager Required materials • Gloves: clean, powder-free, chemical-resistant • Replacement ferrule To replace the ferrule on the column fitting: Unscrew the cap nut from the fitting. Cap nut Fitting Slide the fitting off of the tubing. Tubing Fitting The ferrule unlocks from the fitting.
  • Page 121 Replacing the ferrule on the column fitting Remove the ferrule from the fitting. Fitting Ferrule Install the new ferrule on the fitting. Fitting Ferrule Slide the fitting and ferrule back onto the tubing. Tubing Fitting The ferrule locks onto the fitting. Result: October 7, 2014, 715004178 Rev.
  • Page 122 3 Maintaining the 30-cm single-zone column manager Screw the cap nut back onto the fitting. Cap nut Fitting October 7, 2014, 715004178 Rev. B...

  • Page 123: Replacing The Valve Cartridge

    Replacing the valve cartridge Replacing the valve cartridge This procedure is only applicable to CM-30S module installed in a UPC Note: system. Before beginning the replacement procedure, ensure that you have the correct part for your system type. To avoid the harmful effects of personal contact with solvents, Warning: including inhalation, observe Good Laboratory Practice when you handle them.
  • Page 124 3 Maintaining the 30-cm single-zone column manager Ensure the tubing connected to the switching-valve is labeled correctly. Valve cartridge: Lock ring Lock ring stop 2-mm hex screw Stator ID Use the 1/4-inch wrench to remove the fittings attached to the switching-valve cartridge.

  • Page 125: Cleaning The Exterior Of The Cm-30S

    Requirement: as the old. It must slide fully into the switching-valve assembly. If it does not, contact Waters Technical Service. 13. Insert the 2-mm hex screw at the 10 o’clock position on the switching-valve cartridge, and tighten the screw using the 2-mm Allen wrench.
  • Page 126 3 Maintaining the 30-cm single-zone column manager October 7, 2014, 715004178 Rev. B...

  • Page 127: A Safety Advisories

    The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device, or a component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or...
  • Page 128 A Safety Advisories procedures, an accompanying statement identifies the applicable risk and explains how to avoid it. (General risk of danger. When this symbol appears on an Warning: instrument, consult the instrument’s user documentation for important safety-related information before you use the instrument.) (Risk of burn injury from contacting hot surfaces.) Warning: (Risk of electric shock.)

  • Page 129: Specific Warnings

    Biohazard warning The following warning applies to Waters instruments and devices that can process material containing biohazards, which are substances that contain biological agents capable of producing harmful effects in humans.

  • Page 130: Notices

    A Safety Advisories Biohazard and chemical hazard warning This warning applies to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. To avoid personal contamination with biohazards, Warning: toxic materials, or corrosive materials, you must understand the hazards associated with their handling.

  • Page 131: Warnings That Apply To All Waters Instruments And Devices

    Warnings that apply to all Waters instruments and devices Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section. Changes or modifications to this unit not expressly approved by the Attention: party responsible for compliance could void the user’s authority to operate the...
  • Page 132 A Safety Advisories Use caution when working with any polymer tubing under pressure: Warning: • Always wear eye protection when near pressurized polymer tubing. • Extinguish all nearby flames. • Do not use tubing that has been severely stressed or kinked. •...
  • Page 133 Warnings that apply to all Waters instruments and devices fare attenzione quando si utilizzano tubi in materiale polimerico Attenzione: sotto pressione: • Indossare sempre occhiali da lavoro protettivi nei pressi di tubi di polimero pressurizzati. • Spegnere tutte le fiamme vive nell'ambiente circostante.
  • Page 134 A Safety Advisories 警告：当有压力的情况下使用管线时，小心注意以下几点： • 当接近有压力的聚合物管线时一定要戴防护眼镜。 • 熄灭附近所有的火焰。 • 不要使用已经被压瘪或严重弯曲的管线。 • 不要在非金属管线中使用四氢呋喃或浓硝酸或浓硫酸。 • 要了解使用二氯甲烷及二甲基亚枫会导致非金属管线膨胀，大大降低管线的耐压能力。 경고: 가압 폴리머 튜브로 작업할 경우에는 주의하십시오. • 가압 폴리머 튜브 근처에서는 항상 보호 안경을 착용하십시오. • 근처의 화기를 모두 끄십시오. • 심하게 변형되거나 꼬인 튜브는 사용하지 마십시오. •...
  • Page 135 Warnings that apply to all Waters instruments and devices The user shall be made aware that if the equipment is used in a Warning: manner not specified by the manufacturer, the protection provided by the equipment may be impaired. L’utilisateur doit être informé que si le matériel est utilisé d’une Attention: façon non spécifiée par le fabricant, la protection assurée par le matériel risque...

  • Page 136: Electrical And Handling Symbols

    A Safety Advisories Electrical and handling symbols Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels. Electrical power on Electrical power off Standby Direct current Alternating current Protective conductor terminal Frame, or chassis, terminal Fuse...

  • Page 137: Handling Symbols

    Electrical and handling symbols Handling symbols The following handling symbols and their associated statements can appear on labels affixed to the packaging in which instruments, devices, and component parts are shipped. Keep upright! Keep dry! Fragile! Use no hooks! October 7, 2014, 715004178 Rev. B...
  • Page 138 A Safety Advisories October 7, 2014, 715004178 Rev. B...

  • Page 139: B Specifications

    Specifications The specifications presented here depend on the conditions in individual laboratories. Refer to the Waters ACQUITY Advanced Polymer Chromatography (APC) System Site Preparation Guide, the ACQUITY ® Site Preparation Guide, or contact the Waters Technical Service organization for additional information about specifications.

  • Page 140: Environmental Specifications

    B Specifications Physical specifications: (Continued) Attribute Specification Right side clearance 7.6 cm min (3.0 inches min) required Left side clearance 7.6 cm min (3.0 inches min) required Environmental specifications The following table lists the environmental specifications for the CM-30S. Environmental specifications: Attribute Specification Operating temperature...

  • Page 141: Performance Specifications

    Performance specifications Electrical specifications: (Continued) Attribute Specification Voltage range 100 to 240 VAC Frequency 50−60 Hz Maximum power draw 600 VA – a. Protection Class I The insulating scheme used in the instrument to protect from electrical shock. Class I identifies a single level of insulation between live parts (wires) and exposed conductive parts (metal panels), in which the exposed conductive parts are connected to a grounding system.
  • Page 142 B Specifications Performance specifications: (Continued) Item Specification Number of columns • Maximum of two selectable banks of columns. Each bank contains a maximum of four columns. • Maximum of eight selectable columns when bypass and waste lines are omitted. • Maximum of six selectable columns with bypass and waste lines in use.
  • Page 143 Performance specifications Performance specifications: (Continued) Item Specification Time to temperature from off state 45 minutes, max. Test conditions: • Column not installed. • No flow. • Measurement taken with internal temperature sensor after 1 hour thermal equilibrium at set point. •...
  • Page 144 B Specifications Performance specifications: (Continued) Item Specification Temperature distribution along +/- 3.0 °C column Test conditions: • Column not installed. • No flow. • Measurement taken relative to compartment air temperature sensor. • Test conducted at 60 °C column compartment temperature. •...

  • Page 145: Wetted Materials Of Construction

    Wetted materials of construction Performance specifications: (Continued) Item Specification Audible noise (per module) <58 dB(A) Test conditions: • Measured at 1 meter standing in front of the CM-30S. • CM-30S in idle mode. • Valves not operating. Wetted materials of construction The following table lists the wetted materials of construction for the CM-30S.
  • Page 146 B Specifications October 7, 2014, 715004178 Rev. B...

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