Making Detector Connections; Preplc Or Delta-Prep System; Connecting Detector Semi-Prep Cells - Waters Prep Series Installation And Maintenance Manual

5. Install compression fittings on each end of the tubing as described in
"Liquid line connections" on page
column connectors.

Making detector connections

This section describes connecting the column outlet to the detector in the
PrepLC and Delta-Prep systems for large-scale and small-scale operation.

PrepLC or Delta-Prep system

Small-scale operation with a Waters 2487 Detector and semi-prep or prep flow
cell requires these preparations:
• A 0.009- or 0.020-inch ID tubing and a union to connect the
SMALL-SCALE OUTLET, on the front of the column rack, to the
detector cell inlet
• An appropriate receptacle in which to put the waste line from the
detector
Large-scale operation with a Waters 2487 Detector requires the appropriate
flow cell (see the
preparations:
• A 0.020-inch ID or 0.040-inch ID tubing to connect the LARGE-SCALE
OUTLET connector to the inlet side of the detector cell
• An appropriate receptacle in which to put the waste line from the
detector
Minimize bandspreading. Keep the tubing length between the column
Tip:
outlet and detector as short as possible by placing the detector on the rack's
center level.

Connecting detector semi-prep cells

The table titled "Flow cell tubing sizes" on page 2-22
which the detector's semi-prep cells are plumbed. Consult it when you connect
to the column outlet.
The type of operation, small-scale or large-scale, can require the sample to
flow on different sides of the detector. When replumbing the column outlet
connection to a detector, make sure you reconfigure the signal source so that it
2-48 Installing the System
table titled "Flow cell tubing sizes" on page
2-21, and tighten the fittings at all
lists the tubing sizes with
2-22) and these