Waters Prep Series Installation And Maintenance Manual page 63

2. Attach an eluent filter or L-shaped tube (both in Startup Kit) to the
solvent line. The tubing must stop against a ridge inside the eluent
filter.
Forcing tubing into the filter by pushing it beyond this ridge
Caution:
can create these problems:
• Reduction of the filter area, which at higher flow rates can starve the
pump of solvent.
• The inlet tube's sharp edge can pare material from the inside the
filter, creating particles that can be sucked into the inlet line. These
particles can foul the gradient proportioning valve, the pump check
valves, the injector, or some other part of the system.
3. Repeat step 1 for the sparge tube for that reservoir.
4. Attach a diffuser to the sparge line.
5. Repeat steps 1 through 4 for all solvent and sparge tubes.
Vent tube connection
Warning:
• Personal harm could result from improperly vented solvent/sparge
reservoirs. Always vent solvent vapors to a fume hood. Use particular
care with a volatile solvent in a cold room, refrigerator, or other
small, sealed environment.
• Sparging organic solvents like acetonitrile and methanol into the
room air releases harmful vapors. If you use organic solvents, run the
solvent reservoirs' vent tubes to an exhaust hood to safely release
vapors.
To connect the vent tube:
1. Cut a length of PTFE tubing (Startup Kit) to extend from a solvent
reservoir to a fume hood. Push one end of the tubing about 1 inch into
the remaining hole in the reservoir cap. This tubing serves as the
reservoir's vent.
Caution:
remains above the solvent level. Otherwise, solvent will be forced
from the reservoir through this vent tube.
Make sure the end of the vent tubing inside the reservoir
Assembling the system 2-29