Waters CORTECS Care And Use Manual
  1. Manuals
  2. Brands
  3. Waters Manuals
  4. Laboratory Equipment
  5. CORTECS
  6. Care and use manual

Waters CORTECS Care And Use Manual

2.7nm columns
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
[ CARE AND USE MANUAL ]
CORTECS and CORTECS Premier 2.7 µm Columns
CONTENTS
I.
INTRODUCTION
II.
III.
IV.
V.
VI.
VII.
I. INTRODUCTION
Thank you for choosing a Waters™ CORTECS™ and/or
CORTECS Premier Column. The CORTECS packing materials
are manufactured in a cGMP, ISO 9001 certified plant using
ultra pure reagents. Each batch of CORTECS packing
material is tested chromatographically with acidic, basic,
and neutral analytes and the results are held to narrow
specification ranges to assure excellent, reproducible
performance. Every column is individually tested and a
Performance Chromatogram and Certificate of Batch
Analysis are provided on the eCord™ Intelligent Chip.
The CORTECS Premier Column is offered with or without
a VanGuard™ Fully-Integrated Technology [FIT] Cartridge.
To address the desire to extend the operating lifetimes of
analytical columns, the VanGuard FIT Cartridge is designed
to prevent the non-desired introduction of sample matrix or
particulates onto the column without degrading the separation.
It can be easily replaced to restore separation performance and
extend the analytical column's lifetime.
In addition, the CORTECS Premier Columns utilize
MaxPeak™ High Performance Surfaces, an innovative
technology designed to increase analyte recovery, sensitivity,
and reproducibility by minimizing analyte/surface interactions
that can lead to sample losses.
1

Advertisement

Table of Contents
loading

Related Manuals for Waters CORTECS

Summary of Contents for Waters CORTECS

  • Page 1: Table Of Contents

    Performance Chromatogram and Certificate of Batch f. CORTECS Columns Analysis are provided on the eCord™ Intelligent Chip. — VanGuard Protection The CORTECS Premier Column is offered with or without g. VanGuard FIT Cartridges a VanGuard™ Fully-Integrated Technology [FIT] Cartridge. III.

  • Page 2: Getting Started

    Note: If (b) is performed, the isocratic efficiencies measured and increase the flow rate to 0.5 mL/min over five minutes. in your laboratory may be less than those given on the Waters “Performance Test Chromatogram.” This is normal. The 3. When the mobile phase is flowing freely from the column...

  • Page 3: Column Qr Code

    2. Determine the number of theoretical plates (N) and use for CORTECS Premier Columns. this value for periodic comparisons. For the CORTECS Premier Columns that have a VanGuard FIT 3. Repeat the test at predetermined intervals to track column Cartridge, the VanGuard FIT Cartridge may be replaced using performance over time.

  • Page 4: Column Use

    Pressure in order to avoid sample and/or buffer precipitation. 1. The 2.1 mm I.D. CORTECS and CORTECS Premier 2.5 µm 4. Filter sample with 0.2 µm membranes to remove particulates. Columns can tolerate operating pressures up to 18,000 If the sample is dissolved in a solvent that contains an organic psi (1241 bar or 124 MPa).
  • Page 5 [ CARE AND USE MANUAL ] Table 2. Buffer Recommendations for Using CORTECS Columns Used for Buffer Volatility Additive/Buffer Mass Comments Range (±1 pH Unit) Spec Ion pair additive, can suppress MS signal, — Volatile used in the 0.02–0.1% range.

  • Page 6: Column Cleaning, Regenerating, And Storage

    Use the cleaning routine that matches the properties of the samples and/or what you believe is contaminating the column (see Table 3). Flush columns with 20-column volumes of Table 3. Recommended pH and Temperature Limits for CORTECS Columns Surface Name of...

  • Page 7: Storage

    Completely seal column to avoid evaporation and drying out of the bed. For periods longer than four days, store CORTECS Columns in 95:5 acetonitrile:water. Do not store in buffered solvent. If the mobile phase contained a buffered salt, flush the column...

  • Page 8: Manufacturing Information

    VanGuard Cartridge. a. Tips for Maximizing CORTECS Column Lifetimes Discourage bacterial growth by minimizing the use ■ 1. To maximize CORTECS Column lifetime, pay close of 100% aqueous mobile phases where possible. attention to: Change aqueous mobile phase every 24–48 hours ■...

  • Page 9: Getting Started With Cortecs Hilic Columns

    ■ HPLC-grade bottled water b. Getting Started with CORTECS HILIC Columns ■ 1. Because CORTECS HILIC columns do not possess a “Topping off” or adding “new” mobile phase ■ bonded phase, the pH operating range is 1–5, and they can to “old”...

  • Page 10: Cautionary Note

    Waters Corporation 34 Maple Street Waters, The Science of What’s Possible, CORTECS, ACQUITY, UPLC, eCord, VanGuard, MaxPeak, Arc, Milford, MA 01757 U.S.A. Oasis, Sep-Pak, Empower, and MassLynx are trademarks of Waters Corporation.

This manual is also suitable for:

Cortecs premier

Table of Contents