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The Replenishment System Will Not Be Used For Some Time - Agilent Technologies 7100 User Manual

Capillary electrophoresis system
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8
Maintenance
Maintenance of the Replenishment System

The Replenishment System Will Not Be Used for Some Time

The replenishment system should be flushed first with water and then with
isopropanol.
Please follow these steps.
1 Open the Lab Advisor or Instrument Utilities software and start monitoring
2 In the tools section, select the CE Control screen and click on Change Bottles to
3 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle
4 Fill a minimum 300 mL of water into the electrolyte bottle. Make sure the
5 Make sure the waste bottle is empty.
6 Cap the bottles tightly.
7 Choose Done in the Change Bottles dialog box. Wait until the pressure has
8 To flush the internal tubing system from the electrolyte bottle to the waste
9 To clean the internal tubing system, including the replenishment, select
10 When requested to do so, place a capped and empty vial into a tray position.
11 Execute the clean level sensing operation to clean the replenishment needle
12 Repeat all steps starting with number 2 and use isopropanol instead of
218
the respective instrument.
release the pressure before you open the bottles.
has a frit and tubing inside. The frit must not get dirty.
water is filtered by using a 0.25 µm or 0.45 µm filter depending on its
viscosity.
built up again.
bottle, including all valves on this pathway, select Flush Tubes. During this
step the replenishment needle itself is not flushed. Set a flushing time of 2
minutes.
Clean Tubes. During this step the replenishment is used to fill and empty a
vial several times. Set the number of repetitions to 5.
entirely.
water. Empty the waste bottle whenever you fill or refill the electrolyte
bottle.
Agilent 7100 Capillary Electrophoresis System User Manual

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