Empty Injection Vial; Dirt In The Vials; Air Bubble In Injection Vial Or Run Buffer Vials; Air Bubble In Capillary - Agilent Technologies 7100 User Manual

Empty Injection Vial

Access the vial position specified in the Sample info or the Sequence table using
the Get vial function in the Tray control screen.
• Check if the vial is filled.

Dirt in the Vials

Clean all vials prior to use. First, flush them with water followed by, for
example, some isopropanol.
• Use compressed air to dry the vials inside.

Air Bubble in Injection Vial or Run Buffer Vials

When filling sample or buffers into the vial, an air bubble is sometimes
formed. This happens very frequently when using microvials. Air bubbles can
cause air to be injected instead of the sample.
• To avoid air bubbles, carefully fill vials starting from the bottom.
• If you cannot avoid the formation of air bubbles, adjust the lift offset in the

Air Bubble in Capillary

Small air bubbles in the capillary may cause spikes in your electropherogram.
Larger air bubbles expand so much during analysis that the current drops
below the lower alarm limit.
• Flush your capillary with buffer to remove air bubbles.
Agilent 7100 Capillary Electrophoresis System User Manual
Home values dialog box so that the capillary doesn't reach into the bubbles.
Troubleshooting and Diagnostics
CE Problem Solving
6
167