Changing The Buffer Composition In The Replenishment System - Agilent Technologies 7100 User Manual

Changing the Buffer Composition in the Replenishment System

Please follow these steps.
1 Open the Lab Advisor or Instrument Utilities software and start monitoring
2 In the Tools section, select the CE Control screen and click on Change Bottles to
3 Take out the electrolyte bottle and unscrew the cap. The electrolyte bottle
4 Fill a minimum 300 mL of buffer into the electrolyte bottle. Make sure the
5 Make sure the waste bottle is empty.
6 Cap the bottles tightly.
7 Choose Done in the Change Bottles dialog box. Wait until the pressure has
8 To flush the internal tubing system from the electrolyte bottle to the waste
9 To clean the internal tubing system, including the replenishment, select
10 When requested to do so, place a capped and empty vial into a tray position.
The involved volume of the replenishment system is about 5 mL. A 10-time
flush is sufficient. The flush time depends primarily on the viscosity of your
buffer. Therefore, choose a short time and repeat flush tubes if necessary,
otherwise it is possible that the buffer is flushed completely into the waste
bottle. This protocol suggests a combination of flush tubes first and a
subsequent clean tubes step, with comparably low volume consumption, for a
high exchange efficiency and low buffer consumption.
Agilent 7100 Capillary Electrophoresis System User Manual
the respective instrument.
release the pressure before you open the bottles.
has a frit and tubing inside. The frit must not get dirty.
buffer is filtered by using a 0.25 µm or 0.45 µm filter depending on its
viscosity.
built up again.
bottle including all valves on this pathway, select Flush Tubes. During this
step the replenishment needle itself is not flushed. Set a flushing time of
0.3 minutes.
Clean Tubes. During this step the replenishment is used to fill and empty a
vial several times. Set the number of repetitions to 5.
Maintenance of the Replenishment System
Maintenance 8
217