Capillary Electrochromatography, Cec; How To Use Your Agilent Ce Instrument For Capillary Electrochromatography - Agilent Technologies 7100 User Manual

5 Using the Agilent 7100 Capillary Electrophoresis System

Capillary Electrochromatography, CEC

Capillary Electrochromatography, CEC

Capillary electrochromatography (CEC) is a fusion of capillary electrophoresis
and capillary chromatography. In CEC, solvent is mobilized through a packed
capillary column by electroosmotic flow instead of the hydraulic flow used in
liquid chromatography LC. Higher efficiencies are achievable because of the
uniform flow velocity profile and the ability to use smaller particle sizes in
longer columns than is possible with LC. Operation of CEC requires an equal
application of high pressure to the inlet and outlet vials in order to suppress
bubble formation while avoiding hydrodynamic flow due to a pressure
differential.
How to Use Your Agilent CE Instrument for Capillary
Electrochromatography
Requirements
• External pressure supply (< 15 bar) of oil-free air or nitrogen.
• Push-fit connector and gas line (supplied in startup kit).
• Outlet lift connection to the tubing system
For external pressure supply, a male adapter fitting the instrument's female adapter and
N O T E
PTFE tubing (1/8" OD x 1/16" ID PTFE tubing) are part in the accessory kit of the
instrument. Please add a suitable connector to the external pressure supply.
The inlet for the external high pressure gas is situated at the back of the
instrument.
148 Agilent 7100 Capillary Electrophoresis System User Manual