Further Action; Operations For Replenishment Maintenance - Agilent Technologies 7100 User Manual

Further Action

If the instrument cannot build-up pressure or a vacuum after performing the
above steps, check the following:
1 Proper seal of the electrolyte bottle
2 Proper seal of the waste bottle
3 Tight fittings
If none of these reasons apply, call Agilent Technologies.

Operations for Replenishment Maintenance

The following operations can be started from the ChemStation, Lab Advisor or
Instrument Utilities software. From the ChemStation, they can be done by
clicking the replenishment vial. It is a vial located between the replenishment
bottles in the CE dialog. From the Lab Advisor or Instrument Utilities
software, start monitoring, visit the Tools section and the CE Control screen.
Flush Tubes
The Flush Tubes operation directly flushes water, buffer or other cleaning
solutions from the electrolyte bottle to the waste bottle. During this task, a
reduced pressure is utilized in the electrolyte bottle. The user can set a time
for this operation.
The volume in the tubing system from the electrolyte bottle and the waste
bottle is about 5 mL. It is recommended that you flush at least with 10-fold
volume of fresh buffer. The exchange flow rate of water is approximately 120
to 150 mL/min. Please note the individual viscosity of your buffer system.
It is important to make sure that there is enough buffer (for example a
minimum of 300 mL) in the electrolyte bottle for the Flush Tubes operation. An
excessive Flush Tubes time will drain the electrolyte bottle, causing air bubbles
to be introduced into the system.
The Flush Tubes command does not include a cleaning of the replenishment
needle. It requires an execution of the Clean Tubes function to clean the last
part to the fill needle of the replenishment needle.
Agilent 7100 Capillary Electrophoresis System User Manual
Maintenance of the Replenishment System
Maintenance 8
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