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4. Press the tab on the cap and push the plunger into the
cartridge until it rests against the top frit.
5. Release the tab on the cap. Note that the opposite end of
the tab secures the cartridge.
6. Load the solid sample cartridge with cap on the sample
injection port.
3.1.3 Preloading on Column
You can load the reaction mixture or sample directly onto the
column, sometimes called "direct" or "dry" loading.
Note
If using this method, use care to ensure that a column equilibration
does not wash away the sample. Bypass the column equilibration
at run time by selecting "Preload on column" as the sample
Loading Type. Refer to Section 3.3 for more details.
3.2 Loading a RediSep R
To load a RediSep R
Note
For best results, always use RediSep R
not automatically detect other columns, including non-Rf RediSep
columns. The system also limits the maximum operating pressure
to 50 psi when the column is not detected.
1. Select a RediSep R
column selection guide that can assist you in selecting a
stationary phase media and column size.
2. Raise the injection valve and insert the column into the top
column fitting. Note that the column fittings are keyed to
ensure the correct flow direction.
3. Slowly lower the injection valve while aligning the bottom
column fitting. The spring-loaded injection valve will hold
the column in place.
4. To seal the column fittings, give the column a slight twist
/
1
(
turn).
4
Column
f
column:
f
column. The on-line help includes a
f
Section 3 Operation
columns. The system will
f
3-3
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