Required Materials; Preparation Process; Generating Target Median Values - Beckman Coulter CytoFLEX Instructions For Use Manual

Required Materials

The following materials are required to complete the QC process:
• CytoFLEX Daily QC Fluorospheres or CytoFLEX Ready to Use Daily QC Fluorospheres, or other
material applicable for your application
• CytoFLEX Daily IR QC Fluorospheres (for systems configured with an IR laser)
• CytoFLEX Sheath Fluid
• Sample tubes (12 x 75 mm).
• Vortexer

Preparation Process

For procedures, refer to
Ready to Use Daily QC Fluorospheres Preparation Process
Fluorospheres Preparation

Generating Target Median Values

1
Double-click
a. Ensure that the
green.
b. If the icon is not green, ensure that the Cytometer USB is securely connected to the
Workstation and restart the Workstation.
2
Select
Open Experiment
standardize.
NOTE
Ensure the Gain and Threshold settings are optimal. Refer to
Threshold
3
Right-click the tube and select
settings.
B49006AS
CytoFLEX Daily QC Fluorospheres Preparation Process
Process.
to start the CytExpert software.
icon on the Status Bar near the bottom-left side of the display is
Connected
to open an experiment with the desired acquisition settings to
in CHAPTER 6, Data Acquisition and Sample
Duplicate without Data
Instrument Quality Control and Standardization
and/or
and/or CytoFLEX Daily IR QC
Adjusting the Gain
Analysis.
to create a tube with the same acquisition
5
Standardization
CytoFLEX
and Adjusting the
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