Beckman Coulter CytoFLEX Instructions For Use Manual page 191

Flow cytometers
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2
Select a QC run from the QC Process list on the left and a QC report appears on the right.
NOTE
The results column indicates a passing QC result with a
QC results must meet the following criteria to pass:
B49006AS
The gain differences must be 20% from the target gain.
The median fluorescence intensity (MFI) differences must be 5% from the target MFI.
The rCV must be within the range.
NOTE
The CytoFLEX Daily QC Fluorospheres / CytoFLEX Ready to Use Daily QC Fluorospheres
/ Daily IR QC Fluorospheres (808 Laser) rCV must meet the following criteria to pass:
The rCV for channels of the 405 nm, 488 nm, 561 nm, 638 nm lasers must be ≤5%.
The rCV for channels of the 355 nm, 375 nm, 808 nm lasers must be ≤7%.
Instrument Quality Control and Standardization
and a failed QC result with
5
Quality Control
.
5-21

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