Setting Collection Conditions - Beckman Coulter CytoFLEX Instructions For Use Manual

Data Acquisition and Sample Analysis
Configuring Acquisition Settings
4
Set the desired threshold using one of the following methods:
• Choose the channel that is used for setting the threshold. Manually enter the threshold
value in the Threshold tab.
NOTE
• Select
to seek the target signal based on the background signal. It can quickly help find the target
population if the signal-to-noise ratio (SNR) of the channel is comparatively good. The
threshold can be set to either "H" (signal height) or "A" (signal area).
NOTE
NOTE
Moreover, "and" as well as "or" can be applied to as many as two channels, so as to allow
these Boolean logical operators to be used in setting the threshold value.
— "and": Data is displayed and collected only when two threshold conditions are met
— "or": Data is displayed and collected when at least one of two threshold conditions are
• Select
position in the desired plot and select once.
5
Select
Close

Setting Collection Conditions

1
Check mark the conditions required to set the necessary stop count events on the left side of
the Acquisition screen.
Three stop count collection conditions are available for sample recording:
•
Events to Record
•
Time to Record
•
Volume to Record
6-44
For dual-parameter plots, you can right-click the plot and select both parameters if desired.
Then, select the desired threshold boundary for the second parameter.
in the Primary Threshold Trigger Level section of the Acq. Setting screen
Automatic
The automatic threshold value is based on the relative signal difference. When adjusting
gain, you do not need to update the threshold settings. For channels with a low SNR or an
excessively impure signal, manually setting the threshold parameters is recommended.
It is recommended to use Manual mode to set the threshold parameters to obtain accurate
experiment data.
simultaneously.
met.
from the plot control area. Move your mouse pointer to the desired threshold
.
. Used to set the number of events to record in the specified population.
. Used to set the collection time duration in seconds.
. Used to set the collection volume in μL.
B49006AS