Preparing The Qc Sample [With Plate Loader]; Required Materials; Preparation Process Cytoflex Daily Qc Fluorospheres - Beckman Coulter CytoFLEX Instructions For Use Manual

Preparing the QC Sample [With Plate Loader]

Required Materials

The following materials are required to complete the QC process:
• CytoFLEX Daily QC Fluorospheres or CytoFLEX Ready to Use Daily QC Fluorospheres
• CytoFLEX Daily IR QC Fluorospheres (for systems configured with an IR laser)
• CytoFLEX Sheath Fluid or another nonionic sheath fluid
• Standard 96-well plate
— 96-well flat-bottom
— 96-well V-bottom
— 96-well U-bottom
• 96-well deep well plate
— 96-well V-bottom
— 96-well U-bottom
• Vortexer

Preparation Process CytoFLEX Daily QC Fluorospheres

IMPORTANT
CytoFLEX Daily IR QC Fluorospheres, refer to
Process
1
Take one 96-well plate and record the QC sample well position.
2
Use the vortexer or shake vigorously to thoroughly mix the bottle of CytoFLEX Daily QC
Fluorospheres.
IMPORTANT
3
Add one drop of CytoFLEX Daily QC Fluorospheres to the sample well.
4
Add 200 L of deionized water to the sample well.
5
Place the well plate in a dark location at 2-8 C until ready to load the well plate into the
instrument for QC.
NOTE
Well plates containing diluted CytoFLEX Daily QC Fluorospheres should be stored sealed in a dark
location at 2-8 C for up to 5 days.
B49006AS
For preparation instructions for the CytoFLEX Ready to Use Daily QC Fluorospheres or the
or CytoFLEX Daily IR QC Fluorospheres Preparation
Do not overfill the sample well.
Instrument Quality Control and Standardization
CytoFLEX Ready to Use Daily QC Fluorospheres Preparation
Process.
5
Quality Control
5-5