Performance Characteristics [Cytoflex Lx] - Beckman Coulter CytoFLEX Instructions For Use Manual

Performance Characteristics [CytoFLEX LX]

Sensitivity
Fluorescence resolution
Blue Side scatter
resolution
Violet Side scatter
resolution
Forward and side scatter
resolution
Carryover
Signal acquisition speed
Carryover
Throughput [With
Standard Plate
a
Loader]
a. This performance characteristic is different if you have the Sample Injection Mode Control Kit installed on your CytoFLEX LX flow cytometer. Refer to
Carryover
Throughput [With
Plate Loader DW]
a. The Plate Loader DW is equipped with the Sample Injection Mode Control Kit. Refer to
B49006AS
MESF
rCV <3%
The CytoFLEX LX Flow cytometer is capable of achieving <3% rCV. Using
CytoFLEX Daily QC Fluorospheres, CytoFLEX Ready to Use Daily QC
Fluorospheres, or Daily IR QC Fluorospheres (for 808 nm Laser) for daily QC,
the pass criteria is ≤5% for the Violet, Blue, Yellow, and Red lasers while the
pass criteria is ≤7% for NUV, UV and IR lasers.
<300 nm
80 nm relative to polystyrene particles
Scatter performance is optimized for resolving lymphocytes, monocytes,
and granulocytes as well as nanoparticles.
Single Tube
Loader format
30,000 particles/second with 23 parameters
Performance [With Standard Plate Loader]
Plate Loader format
10 second acquisition without mixing or backflush: <34 min.
10 second acquisition with 3 second mixing and 3 second backflush: < 47 min.
Performance [With Plate Loader DW]
Plate Loader format
Standard 96-well plate, 10 second acquisition without mixing or backflush: <39
min.
a
Deep-well 96-well plate, 10 second acquisition without mixing or backflush: <40
min
Standard 96-well plate, 10 second acquisition with 5 second mixing and 6 second
backflush: <60 min.
Deep-well 96-well plate, 10 second acquisition with 10 second mixing and 6
second backflush: <69 min.
Performance
FITC: <30 molecules of equivalent soluble fluorochrome
(MESF-FITC)
PE: <10 molecules of equivalent soluble fluorochrome
(MESF-PE)
APC: <25 molecules of equivalent soluble fluorochrome
(MESF-APC)
≤1.0%
≤1.0%
≤1.0%
APPENDIX C, Sample Injection Mode Control Kit.
System Overview
Performance Characteristics
APPENDIX C, Sample Injection Mode Control Kit.
1-43
1