Beckman Coulter CytoFLEX Instructions For Use Manual page 145

Flow cytometers
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WARNING
Risk of chemical injury from bleach. To avoid contact with the bleach, use barrier
protection, including protective eyewear, gloves, and suitable laboratory attire.
Refer to the Safety Data Sheet for details about chemical exposure before using
the chemical.
3
If necessary, empty all waste liquid from the waste container. If biohazardous samples are used
for data collection, add 400 mL of 5 to 6% bleach to the waste container. Refer to
4 L Waste Container
4
Verify that the Fluid Containers and the Cytometer are on the same level.
5
Verify that all sheath fluid tubing, waste tubing, and sensor cables are properly connected, as
shown in the figure:
1. Waste level sensor connector. Connects to the waste liquid sensor cable.
2. Flow cell waste out. Connects to the flow cell waste tubing.
3. Sheath fluid level sensor connector. Connects to the sheath fluid sensor cable.
4. Waste out. Connects to the waste liquid tubing.
5. Sheath return. Connects to the sheath fluid tubing.
6. Sheath fluid in. Connects to the sheath fluid tubing.
B49006AS
in
CHAPTER 12, Replacement/Adjustment
b
c
e
f
Procedures.
d
g
4
Daily Startup
Pre-Startup Inspection
Emptying the
4-3

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