Beckman Coulter CytoFLEX Instructions For Use Manual page 307

Defining the Negative Population Using Unstained Samples
1
Confirm that the instrument has been initialized. Refer to
CHAPTER 4, Daily
CAUTION
Risk of erroneous results. Calculations based on excessively small volumes of
sampled data can be inaccurate. Ensure that more than 1,000 positive events and
more than 1,000 negative events are sampled. If the ratio of positive cells is
comparatively low, increase the number of acquisition events to a suitable
amount.
2
Import the gain setting and apply the setting to all tubes. Refer to
CHAPTER 6, Data Acquisition and Sample
that the sample signal appears in a suitable position. Adjust the gate so that it encloses the
target cell population (see
Analysis).
3
Place the negative control tube in the sample tube holder.
4
Select the unstained tube.
5
Select
Run
B49006AS
Startup.
Creating Plots and Gates
to load the sample.
Creating a Compensation Experiment
Initializing the Instrument
Adjusting the Gain
Analysis. Use the pan tool to adjust the axis scale so
in CHAPTER 6, Data Acquisition and Sample
7
Compensation
in
in
7-5