Sample Injection Mode Control - Beckman Coulter CytoFLEX Instructions For Use Manual

Introduction
About this Manual
CHAPTER 3, Operation Principles
Describes how the Cytometer measures scattered light and fluorescence as cells pass through the
laser beam.
CHAPTER 4, Daily Startup
Provides instructions for starting your CytoFLEX flow cytometer and navigating to the sample
testing standby state.
CHAPTER 5, Instrument Quality Control and Standardization
Provides instructions for performing daily quality control (QC) on your CytoFLEX flow cytometer to
confirm the instrument is working correctly and to ensure accurate experimental data
measurement. Quality control allows you to determine whether your instrument can provide
adequate signal strength and precision.
CHAPTER 6, Data Acquisition and Sample Analysis
Provides instructions for operating the CytoFLEX instrument, including data acquisition, analyzing
and exporting results, and manually adjusting the compensation during the acquisition and
analysis.
CHAPTER 7, Compensation
Describes how to create a compensation experiment and automatically calculate compensation
values after acquiring the single color data. It also explains how to use these calculations for other
experiments.
CHAPTER 8, Data Review
Describes how to use the Analysis screen to analyze data that has already been acquired.
CHAPTER 9, Daily Shutdown
Describes how to keep the instrument in optimal condition through daily cleaning during the
shutdown procedure.
CHAPTER 10, Troubleshooting
Describes some common problems and their solutions in a basic troubleshooting matrix.
CHAPTER 11, Cleaning Procedures
Describes how to carry out certain routine and nonscheduled cleaning procedures.
CHAPTER 12, Replacement/Adjustment Procedures
Describes how to carry out certain routine and nonscheduled replacement and adjustment
procedures.
APPENDIX A, Instrument Installation
Provides the instrument installation procedures for your CytoFLEX flow cytometer.
APPENDIX B, CytExpert Electronic Record Management
Provides the instructions for using the CytExpert Electronic Record Management software option.
APPENDIX C, Sample Injection Mode Control Kit
Provides the instructions for using the CytoFLEX Sample Injection Mode Control Kit.
APPENDIX D, Deep Well Plate
Provides a list of deep well plates suggested for use on the Plate Loader DW.
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B49006AS