Beckman Coulter CytoFLEX Instructions For Use Manual page 346

Flow cytometers
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Troubleshooting
Troubleshooting Table
Table 10.1 Troubleshooting (Continued)
Problem
The connection indicator
light in the lower left
corner of the software
screen is red and
displays Disconnected
and Error.
The alarm does not
sound when the waste
container is full or the
sheath fluid container is
low and the software
status display is red.
10-10
Probable Cause
• Data connection error
• The Cytometer is not turned
on.
• The Cytometer's power cable
is disconnected.
• The alarm is not working.
• Instrument data connection
error.
• The sheath fluid/waste
harness float is restricted.
• The sheath fluid/waste
harnesses have been
secured on the wrong
container.
Corrective Action
1. Ensure that the USB data cable is
securely connected to the back of the
Cytometer and the back of the
Workstation. Refer to
Figure
2. Restart the software. Restart the
Workstation. Refer to
Initializing the
Instrument
in
CHAPTER 4, Daily
3. Turn on the Cytometer using the power
switch on the back of the instrument.
4. Verify that the power cable is securely
connected to the back of the Cytometer.
5. If the problem persists,
1. Ensure the sheath fluid/waste harnesses
are secured to the correct container.
2. Ensure that the USB data cable is
securely connected to the back of the
Cytometer and the back of the
Workstation. Refer to
Figure
3. Restart the Workstation. Refer to
Initializing the Instrument
Daily
Startup.
4. Restart the software.
WARNING
Risk of biohazardous contamination if
you have skin contact with the waste
container, its contents, and its
associated tubing. The waste container
and its associated tubing could contain
residual biological material and must be
handled with care. Clean up spills
immediately. Dispose of the contents of
the waste container in accordance with
your local regulations and acceptable
laboratory procedures.
5. Verify that the float of the sensor in the
sheath fluid/waste container moves
freely.
6. Replace the sheath fluid/waste harness.
Refer to
Replacing the Sheath Fluid
Harness and/or Waste Harness
CHAPTER 12, Replacement/Adjustment
Procedures.
7. If the problem persists,
1.22.
Startup.
contact
us.
1.22.
in
CHAPTER 4,
in
contact
us.
B49006AS

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