Preparing The Compensation Sample; Using Control Samples To Generate The Compensation Matrix - Beckman Coulter CytoFLEX Instructions For Use Manual

Compensation
Creating a Compensation Experiment
4
Select
OK
After confirmation, the software automatically generates the following compensation
experiment.
NOTE
Select Area to calculate compensation based on the Area measured. Alternatively, select Height
to calculate compensation based on the Height measured.

Preparing the Compensation Sample

To perform a compensation experiment, prepare:
• A single positive control tube for each color present in the panel
A negative control tube (optional)
•
NOTE
A negative control tube is required if a single positive control tube does not contain a negative
population.
For the negative control sample and single positive control sample, you can use blood, cells, or
dedicated compensation beads such as VersaComp Antibody Capture Beads. For details, refer to the
appropriate reagent instructions for use. The negative control tube is used to determine the
autofluorescence of the sample.

Using Control Samples to Generate the Compensation Matrix

The design of CytoFLEX system makes Gain independent compensation possible. CytExpert
software automatically recalculates the compensation matrix according to gain. Manual
adjustment may be applied but not necessary because it may introduce inaccurate results.
7-4
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B49006AS