Beckman Coulter CytoFLEX Instructions For Use Manual page 319

Flow cytometers
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Both methods allow you to apply the gain independent compensation capability, i.e. importing
the compensation values with or without recalculating the compensation matrix based on the
gain settings.
4
After opening the desired compensation file, the Import Compensation window appears. Select
one of the following:
• Import compensation matrix and convert it with current gains.
• Import compensation matrix.
• Import compensation matrix and gain.
NOTE
5
Select
OK
6
If necessary, select
7
Select
Close
B49006AS
If the tube does not have any data when importing compensation values calculated from other
instrument settings, the software prompts you to select whether the gain settings must be
imported as well. Select Yes to import fluorescence channel gains settings along with the rest
of the data. Select No to allow the CytExpert software to adjust the compensation matrix values
based on the current gain settings.
If the tube does have data when importing compensation values from other instrument settings,
the software prompts you to select whether the compensation values are adjusted based on the
current gain settings.
It is important to note that automatic adjustments to compensation values calculated from
other instrument gain settings could result in incorrect compensation. Always review the data
after importing compensation values to ensure the sample is compensated properly.
.
to apply the compensation values to the selected test tubes.
Apply to
.
7
Compensation
Adjusting Compensation
7-17

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