7.
Carefully remove the new borosilicate glass capillary from its case by
lifting vertically while pressing on the foam with two fingers.
Foam
8.
Load sample into the capillary using a fused silica syringe needle or a
GELoader tip, minimizing any bubbles between the capillary tip and the
sample.
Recommendation:
capillary in half, scoring it with a fused silica cutter so that the
GELoader can reach the capillary's tip.
9.
Thread the knurled nut and approximately 5 mm of conductive
elastomer over the blunt end of the capillary
10. Fit the capillary into the holder (probe).
11. Finger-tighten the nut so that 5 mm of glass capillary protrude from its
end.
Measure the protrusion from the end of the nut to the shoulder of
Tip:
the glass capillary.
When using a GELoader tip, break the glass
Capillary
NanoFlow source
3-21