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Waters Xevo TQ-S micro IVD Overview And Maintenance Manual

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Waters Xevo TQ-S
micro IVD
Overview and Maintenance Guide
715004599IVD/Revision C
Copyright © Waters Corporation 2020 Rev. C
All rights reserved

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Summary of Contents for Waters Xevo TQ-S micro IVD

  • Page 1 Waters Xevo TQ-S micro IVD Overview and Maintenance Guide 715004599IVD/Revision C Copyright © Waters Corporation 2020 Rev. C All rights reserved...
  • Page 2 January 30, 2020, 715004599IVD Rev. C Page ii...

  • Page 3: General Information

    General Information Copyright notice © 2015 - 2020 WATERS CORPORATION. PRINTED IN THE UNITED STATES OF AMERICA AND IN IRELAND. ALL RIGHTS RESERVED. THIS DOCUMENT OR PARTS THEREOF MAY NOT BE REPRODUCED IN ANY FORM WITHOUT THE WRITTEN PERMISSION OF THE PUBLISHER.

  • Page 4: Contacting Waters

    Contacting Waters Contact Waters with technical questions regarding the use, transportation, removal, or disposal of any Waters product. You can reach us via the Internet, telephone, or conventional mail. Waters contact information Contacting medium Information Internet The Waters Web site includes contact information for Waters locations worldwide.

  • Page 5: Considerations Specific To The Xevo Tq-S Micro Ivd

    To prevent ignition of flammable solvent vapors, Nitrogen gas must flow continuously through the mass spectrometer’s ion source when solvents are flowing into the source. The Xevo TQ-S micro IVD monitors the nitrogen gas input pressure, and MassLynx displays a nitrogen gas fail warning if the pressure falls below 400 kPa (4 bar, 58 psi).

  • Page 6: High Temperature Hazard

    Overload hazard Warning: To prevent personal injury, ensure equipment placed on top of the Xevo TQ-S micro IVD does not exceed 15kg. High temperature hazard Warning: To avoid burn injuries, before performing maintenance operations that involve handling components inside the mass spectrometer's ion source, allow the source interior to cool.

  • Page 7: Bottle Placement Prohibition

    The need to decontaminate other vacuum areas of the instrument depends on the kinds of samples the instrument analyzed and their levels of concentration. Do not dispose of the instrument or return it to Waters for repair until the authority responsible for approving its removal from the premises specifies the extent of decontamination required and the level of residual contamination permissible.

  • Page 8: Equipment Misuse Notice

    Equipment misuse notice If equipment is used in a manner not specified by its manufacturer, protections against personal injury inherent in the equipment’s design can be rendered ineffective. Safety advisories Consult Appendix A for a comprehensive list of warning advisories and notices. Operating this instrument When operating this instrument, follow standard quality-control (QC) procedures and the guidelines presented in this section.

  • Page 9: Audience And Purpose

    Operators of the Xevo TQ-S Micro IVD should be professionally trained and qualified laboratory personnel. Intended use of the Xevo TQ-S micro IVD Waters designed the Xevo TQ-S micro IVD for use with the ACQUITY UPLC I-Class / Xevo TQ-S micro IVD System to perform mass spectrometry for in vitro diagnostic use.

  • Page 10: Calibrating

    ACQUITY UPLC I-Class / Xevo TQ-S micro IVD System Guide. Quality control The following quality controls are required when using Waters in vitro diagnostic (IVD) LC-MS/MS Systems. To prevent incorrect reporting of results, verify proper sample vial placement and positioning against the sample list, prior to sample injection.

  • Page 11: Emc Considerations

    Analytical methods for use in reporting clinical results should be evaluated and validated according to local regulations and should conform to internationally recognized guidelines, for example, Clinical and Laboratory Standards Institute guideline C50-A, Mass Spectrometry in the Clinical Laboratory: General Principles and Guidance;...

  • Page 12: Australian Sponsor Information

    Australian sponsor information Australian Sponsor Emergo Australia Level 20, Tower II Darling Park 201 Sussex Street Sydney, NSW 2000 Australia January 30, 2020, 715004599IVD Rev. C Page xii...

  • Page 13: Table Of Contents

    Australian sponsor information............xii 1 Specifications and Operating Modes ........19 Uses and compatibility ................19 1.1.1 ACQUITY UPLC I-Class / Xevo TQ-S micro IVD system ......21 1.1.2 Software and data system ..............21 Ionization source ..................22 IntelliStart Fluidics system ................. 22 1.3.1...
  • Page 14 Ion optics ....................24 MS operating modes ................. 24 MS/MS operating modes ................25 1.6.1 Product (daughter) ion mode ............25 1.6.2 Precursor (parent) ion mode ............. 26 1.6.3 Multiple reaction monitoring mode ............. 26 1.6.4 Constant neutral loss mode............... 27 Sample inlet ....................
  • Page 15 3 Installing and removing the ESI probe ........37 Installing the ESI probe ................37 Removing the ESI probe ................39 4 Maintenance Procedures ............41 Maintenance schedule ................41 Spare parts ....................43 Troubleshooting with Connections INSIGHT ..........43 Safety and handling ..................
  • Page 16 Specific warnings ................112 Notices ....................114 Bottles Prohibited symbol ................ 114 Required protection ................115 Warnings that apply to all Waters instruments and devices ......115 Warnings that address the replacing of fuses ..........118 Electrical and handling symbols ..............120 A.7.1 Electrical symbols................
  • Page 17 A.7.2 Handling symbols ................120 B External Connections ............. 123 External wiring and vacuum connections ............ 123 Connecting the oil-filled roughing pump ............. 124 B.2.1 Connecting electric cables to the oil-filled roughing pump ....127 Connecting the Edwards oil-free roughing pump ......... 128 B.3.1 Connecting electric cables to the Edwards -free roughing pump ...
  • Page 18 January 30, 2020, 715004599IVD Rev. C Page xviii...

  • Page 19: Specifications And Operating Modes

    1.1 Uses and compatibility The Waters Xevo TQ-S micro IVD is a tandem quadrupole, atmospheric pressure ionization (API) mass spectrometer. Designed for routine UPLC/MS/MS analyses in quantitative applications, it can operate at fast acquisition speeds compatible with UltraPerformance LC applications.

  • Page 20: Intellistart Technology

    Figure 1–1: Xevo TQ-S micro IVD shown with visor down, and visor up Visor up TP03407 1.1.0.1 IntelliStart technology IntelliStart™ technology monitors LC/MS/MS performance and reports when the instrument is ready for use. The software automatically tunes and mass calibrates the instrument, displays performance readbacks, and enables simplified setup of the system for use in routine analytical and open access applications.

  • Page 21: Acquity Uplc I-Class / Xevo Tq-S Micro Ivd System

    Help for further details of IntelliStart. 1.1.1 ACQUITY UPLC I-Class / Xevo TQ-S micro IVD system The Waters Xevo TQ-S micro IVD is compatible with the ACQUITY UPLC I-Class IVD system. The ACQUITY UPLC I-Class / Xevo TQ-S micro IVD system includes a binary solvent manager, sample manager (flow-through-needle), column heater with active pre-heating, and a specialized ACQUITY UPLC column.

  • Page 22: Ionization Source

    See the online Help for the Instrument Console system for details. 1.2 Ionization source The Xevo TQ-S micro IVD uses an electrospray ionization (ESI) source. In electrospray ionization, a strong electrical charge is applied to the eluent as it emerges from a nebulizer. The droplets that compose the resultant aerosol undergo a reduction in size (solvent evaporation).

  • Page 23: System Operation

    • From two integral reservoirs. Use standard reservoir bottles (15-mL) for instrument setup and calibration. Use 1.5-mL, low-volume vials (sold separately) to infuse smaller volumes (see page 33). The reservoirs can also deliver sample through direct or combined infusion to enable optimization at analytical flow rates.

  • Page 24: Ion Optics

    1.4 Ion optics The mass spectrometer’s ion optics operate as follows: Samples from the LC or Intellistart fluidics system are introduced at atmospheric pressure into the ionization source. The ions pass through the sample cone into the vacuum system. The ions pass through the transfer optics (StepWave and second ion guide) to the quadrupole.

  • Page 25: Ms/Ms Operating Modes

    Table 1–1: MS operating modes Operating mode Collision cell Pass all masses Resolving (static) In MS mode, used for instrument tuning and calibration before an MS/MS analysis, the instrument can acquire data at scan speeds as high as 10,000 Da/s. See the mass spectrometer’s online Help for further information.

  • Page 26: Precursor (Parent) Ion Mode

    Figure 1–5: Product ion mode Collision cell Static (at precursor mass) Fragmenting Scanning precursor ions and passing all masses 1.6.1.1 Typical applications Product ion mode is typically used for the following applications: • Method development for MRM screening studies: – Identifying product ions for use in MRM transitions.

  • Page 27: Constant Neutral Loss Mode

    1.6.3.2 RADAR In RADAR mode the Xevo TQ-S micro IVD rapidly alternates between MRM and full scan MS acquisition modes. The instrument tracks target analytes with precision in MRM mode, while at the same time scanning (in MS mode) the background for all other components.

  • Page 28: Sample Inlet

    1.8 Leak sensors Leak sensors in the Xevo TQ-S micro IVD and the drip trays of the ACQUITY UPLC system continuously monitor system components for leaks. A leak sensor stops system flow when its optical sensor detects about 1.5 mL of accumulated, leaked liquid in its surrounding reservoir.

  • Page 29: Vacuum System

    1.9 Vacuum system An external roughing pump and an internal split-flow turbomolecular pump combine to create the source vacuum. The turbomolecular pump evacuates the analyzer and ion transfer region. Vacuum leaks and electrical or vacuum pump failures cause vacuum loss, the damage from which is prevented by protective interlocks.
  • Page 30 January 30, 2020, 715004599IVD Rev. C Page 30...

  • Page 31: Preparing For Operation

    IP addresses of the system instruments. • In an ACQUITY UPLC I-Class / Xevo TQ-S micro IVD System, you must power on the Sample Manager prior to the mass spectrometer. See the mass spectrometer’s online Help for details on MassLynx and IntelliStart software.

  • Page 32: Verifying The Instrument's State Of Readiness

    • During initialization, the binary solvent manager’s and sample manager’s status LED flashes green. • After the instruments successfully power-on, all power LEDs show steady green. The binary solvent manager’s flow LED, the sample manager’s run LED, and the mass spectrometer’s Operate LED remain off. Start the MassLynx software, and monitor the Instrument Console software for messages and LED indications.

  • Page 33: Tuning And Calibration Information

    2.1.4 Running the instrument at different flow rates The ACQUITY UPLC system runs at high flow rates. To optimize desolvation, and thus sensitivity, run the ACQUITY Xevo TQ-S micro IVD system at appropriate gas flows and desolvation temperatures. IntelliStart software automatically sets these parameters when you enter a flow rate, according to the following table.

  • Page 34: Purging The Infusion Pump

    Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while installing reservoir bottles. To install the reservoir bottles: Remove the reservoir bottle caps. Screw the reservoir bottles onto the instrument, as shown below. TP03410 For each reservoir bottle, ensure that the end of the solvent delivery tube is positioned so that it is close to, but does not touch, the bottom of the bottle.

  • Page 35: Rebooting The Instrument

    • Depending on the solutions used, the instrument’s solvent delivery system can require more than one purge cycle to minimize carryover. • To prevent crystallisation in the fluidics system when using salt solutions, always flush the fluidics system with solvent after use. 2.3 Rebooting the instrument The reset button causes the mass spectrometer to reboot.

  • Page 36: Emergency Shutdown Of The Mass Spectrometer

    In these instances, put the mass spectrometer in Standby mode. See the online Help for details. 2.4.1 Emergency shutdown of the mass spectrometer To shut down the instrument in an emergency: Warning: To avoid electric shock, isolate the instrument observing the procedure outlined below.

  • Page 37: Installing And Removing The Esi Probe

    Installing and removing the ESI probe This chapter describes how to install and remove the ESI (electrospray ionization) probe. 3.1 Installing the ESI probe 3.1.0.1 Required material Chemical-resistant, powder-free gloves Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves while performing this procedure.
  • Page 38 With the probe label facing you, carefully slide the ESI probe into the hole in the probe adjuster assembly, ensuring that the probe location dowel aligns with the location hole of the probe adjuster assembly. Probe label Probe locking ring Probe location dowel Location hole of the probe adjuster assembly...

  • Page 39: Removing The Esi Probe

    • If you are replacing the tubing between the selector valve and the probe, minimize the length to reduce peak broadening. • When cutting the tubing to length, cut it squarely (that is, perpendicular to its horizontal axis). 3.2 Removing the ESI probe 3.2.0.1 Required material Chemical-resistant, powder-free gloves...
  • Page 40 January 30, 2020, 715004599IVD Rev. C Page 40...

  • Page 41: Maintenance Procedures

    Maintenance Procedures This chapter provides the maintenance guidelines and procedures necessary to maintain the instrument’s performance. Keep to a maintenance schedule, and perform maintenance as required and described in this chapter. 4.1 Maintenance schedule The following table lists periodic maintenance schedules that ensure optimum instrument performance.
  • Page 42 XDS46i Instruction Manual A731-01-880. Replace the dry roughing pump’s 35000 hours. Contact Edwards bearings. Replace the turbo pump bearings Every 4 years. Contact Waters and oil. Clean the source components. When they are page visibly fouled, the background or high-peak...

  • Page 43: Spare Parts

    4.2 Spare parts Replace only spare parts, which are the parts mentioned in this document. For details about spare parts, use the Waters Quality Parts Locator on the Waters Web site’s Services & Support page. 4.3 Troubleshooting with Connections INSIGHT Connections INSIGHT is an “intelligent”...

  • Page 44: Safety And Handling

    4.4 Safety and handling Bear in mind the following safety considerations when performing maintenance procedures: Warning: To avoid personal contamination, always wear chemical-resistant, powder-free gloves while handling instrument components. The components can be contaminated with biohazards or toxic materials. Warning: To avoid skeletal or muscle injury associated with lifting heavy objects, use suitable machinery and the supplied harness to lift the mass spectrometer.

  • Page 45: Removing And Refitting The Source Enclosure

    In the Instrument Console, click Stop Flow to stop the LC flow or, if column flow is required, divert the LC flow to waste as follows: In the Instrument Console system tree, expand Xevo TQ-S micro IVD, Interactive Fluidics. Click Control Select Waste as the flow state.

  • Page 46: Fitting The Source Enclosure To The Instrument

    Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure. Using both hands, grasp the source enclosure and lift it vertically off the two supporting studs on the source adaptor housing. Cable storage positions Supporting stud TP03164 Source enclosure...

  • Page 47: Operating The Source Isolation Valve

    4.7 Operating the source isolation valve You must close the source isolation valve to isolate the source from the instrument vacuum system for certain maintenance procedures. 4.7.0.1 Required material Chemical-resistant, powder-free gloves Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.
  • Page 48 Close the source isolation valve by moving its handle counterclockwise, to the vertical position. Isolation valve handle in closed position Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.

  • Page 49: Removing O-Rings And Seals

    To open the source isolation valve after completing a maintenance procedure: Open the source isolation valve by moving its handle clockwise to the horizontal position. Isolation valve handle in open position Close the source enclosure. 4.8 Removing O-rings and seals When performing certain maintenance procedures, you must remove O-rings or seals from instrument components.

  • Page 50: Cleaning The Instrument Case

    Figure 4–1: O-ring removal kit Tool 1 Tool 2 Notice: To avoid scratching the O-ring or seal, take care while using the removal tools to remove it from components. To remove an O-ring: Use the forked end of tool 1 to impale the O-ring or seal. Pull the O-ring or seal from its groove;...
  • Page 51 Figure 4–2: Nitrogen exhaust trap bottle To laboratory exhaust (12-mm) From instrument Pilot valve tubing waste (12-mm) 4.10.0.1 Required material Chemical-resistant, powder-free gloves To empty the nitrogen exhaust trap bottle: In the MassLynx software’s MS Console, click Stop Flow Pull the source enclosure release (located at the bottom, right-hand side) outward, and swing open the enclosure.

  • Page 52: Gas Ballasting The Oil-Filled Roughing Pump

    Warning: To avoid spreading contamination, dispose of the waste liquid in accordance with local environmental regulations. Dispose of the waste liquid in accordance with local environmental regulations. Fit and fully tighten the nitrogen exhaust trap bottle to the cap. Secure the nitrogen exhaust trap bottle in the upright position. Close the source enclosure.

  • Page 53: Inspecting The Roughing Pump Oil Level

    • With ESI operation, once a week • When the roughing pump oil appears cloudy • When the vacuum pressure is higher than normal • When condensate forms in the roughing pump exhaust line • When you change the roughing pump oil Warning: To avoid burn injuries, take great care while working with the roughing pump;...

  • Page 54: Adding Oil To The Roughing Pump

    The roughing pump oil level appears in the roughing pump’s oil level sight glass. Check the oil level weekly; you must maintain the oil level at or near the indicated maximum level when the pump is not operating. Requirement: You must check the oil level while the roughing pump is running. Note that the oil level in the sight glass is lower when the pump is running than when it is stopped.

  • Page 55: Replacing The Roughing Pump Oil

    Notice: To avoid oil leakage, and consequent damage to the pump, when refitting the oil filler plug, ensure that: • the plug is not cross-threaded; • the O-ring is not pinched; • the plug is not overtightened. Use the 8-mm Allen wrench to fit and tighten the roughing pump’s oil filler plug. Tip: When the oil filler plug is tightened, the plug seals by means of an O-ring.
  • Page 56 Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when adding or replacing oil. The roughing pump oil can be irritant, or contaminated with biohazardous or toxic analyte accumulated during normal operation.

  • Page 57: Replacing The Roughing Pump's Oil Demister Element

    Tip: When the oil drain plug is tightened, the plug seals by means of an O-ring. Compression is controlled by the O-ring groove depth in the plug. Increased torque does not improve the plug seal; it only makes the plug difficult to remove later.
  • Page 58 Allow the roughing pump to cool. Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when adding or replacing oil. The roughing pump oil can be irritant, or contaminated with biohazardous or toxic analyte accumulated during normal operation.
  • Page 59 Use the 10-mm wrench to remove the nut that secures the oil demister element to the exhaust flange. Spring Securing nut TP02686 Holding the oil demister element slightly elevated to prevent the loss of the spring, remove the exhaust flange from the oil demister element. TP02692 Remove the spring from the oil demister element.
  • Page 60 Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when replacing the oil demister element. The pump oil can be irritant, or contaminated with biohazardous or toxic analyte accumulated during normal operation.

  • Page 61: Cleaning The Source Components

    Notice: To avoid damage, do not overtighten the nut that secures the oil demister element onto the exhaust flange; ensure that only approximately 1 mm of thread is exposed beyond the nut when it is tightened. Use the 10-mm wrench to fit and tighten the nut that secures the oil demister element to the exhaust flange.

  • Page 62: Removing The Sampling Cone Assembly From The Source

    4.17.1 Removing the sampling cone assembly from the source 4.17.1.1 Required material Chemical-resistant, powder-free gloves Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components. Warning: To avoid electric shock, before beginning this procedure, prepare the instrument according the procedure on...

  • Page 63: Disassembling The Sampling Cone Assembly

    Slide the sampling cone assembly out of the ion block assembly. Sampling cone Ion block assembly assembly 4.17.2 Disassembling the sampling cone assembly 4.17.2.1 Required material • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when...
  • Page 64 Insert the collar in the sample cone. Notice: To avoid damaging the fragile sample cone, do not place it on its tip; instead, place it on its flanged base. Rotate the tool and collar counter-clockwise and then lift them to remove the sample cone from the cone gas nozzle.

  • Page 65: Cleaning The Sample Cone And Cone Gas Nozzle

    Remove the O-ring from the sample cone. Cone gas nozzle Cone gas nozzle handle O-ring Warning: To avoid spreading contamination, dispose of the O-ring or seal in accordance with local environmental regulations. If the O-ring shows signs of deterioration or damage, dispose of it in accordance with local environmental regulations.

  • Page 66: Assembling The Sampling Cone Assembly

    Notice: To avoid damaging the fragile sample cone, do not place it on its tip; instead, place it on its flanged base. To clean the sample cone and cone gas nozzle: If the sample cone contains debris, place a drop of formic acid on its aperture. Immerse the sample cone, cone gas nozzle, and cone gas nozzle handle in separate glass vessels containing 1:1 methanol/water.

  • Page 67: Fitting The Sampling Cone Assembly To The Source

    To assemble the sampling cone assembly: Fit the cone gas nozzle handle onto the cone gas nozzle and turn the handle clockwise to tighten. O-ring Sample cone Cone gas nozzle TP02663 Cone gas nozzle handle Carefully fit the sample cone into the cone gas nozzle. Fit the O-ring into the groove created between the sample cone and cone gas nozzle.

  • Page 68: Cleaning The Ion Block Assembly

    Hold the sampling cone assembly so that the cone gas nozzle handle is oriented horizontally and at the top, and then slide the sampling cone assembly into the ion block assembly. Ion block assembly Sampling cone Grasp the cone gas nozzle handle and use it to rotate the sampling cone assembly 90 degrees, moving the handle downward from the horizontal to the vertical position.
  • Page 69 To remove the ion block assembly: Warning: The source components can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Vent and shut down the mass spectrometer (see the mass spectrometer’s online Help for details). Warning: To avoid personal injury, as well as damage to the roughing pumps and mass spectrometer, disconnect the power cords for the mass...

  • Page 70: Disassembling The Source Ion Block Assembly

    Remove the ion block assembly from the PEEK ion block support. PEEK ion block support Ion block assembly 4.18.2 Disassembling the source ion block assembly 4.18.2.1 Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool •...
  • Page 71 To disassemble the ion block assembly: Warning: The ion block assembly can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves to perform this procedure. Ensure that the source isolation valve is closed. Source isolation valve handle in closed position Sampling cone assembly retaining...
  • Page 72 Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate. Ion block cover plate securing screw Ion block cover plate Remove the ion block cover plate. Grasp the isolation valve, and pull it out of the ion block. Isolation valve O-ring Use the O-ring removal kit to carefully remove the isolation valve O-ring (see...
  • Page 73 Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. Heater cartridge assembly wires PEEK terminal block securing screw Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block.

  • Page 74: Cleaning The Ion Block Components

    11. Use the O-ring removal kit to carefully remove the cover seal from the ion block (see also page 49). Cover seal Cone gas O-ring 12. Use the O-ring removal kit to carefully remove the cone gas O-ring from the ion block.

  • Page 75: Assembling The Source Ion Block Assembly

    To clean the ion block components: Warning: The ion block components can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Warning: Formic acid is extremely corrosive and toxic. Work with extreme care, use a fume hood and suitable protective equipment. Immerse the ion block and isolation valve in separate glass vessels containing 1:1 methanol/water.

  • Page 76: Fitting The Ion Block Assembly To The Source Assembly

    • Lint-free cloth To assemble the ion block assembly: Notice: • To avoid recontaminating the ion block assembly, wear clean, chemical-resistant, powder-free gloves during this procedure. • To avoid damaging the heater cartridge assembly wires, do not bend or twist them when fitting the assembly and ceramic heater mounting block to the ion block.

  • Page 77: Cleaning The Ion Guide Assembly

    To fit the ion block assembly to the source assembly Warning: The source components can be contaminated with biohazardous and/or toxic materials. Always wear chemical-resistant, powder-free gloves while performing this procedure. Warning: To avoid puncture wounds, take great care while working with the source enclosure open if an ESI probe is fitted (the probe tip is sharp).

  • Page 78: Removing The Ion Guide Assembly And Differential Aperture From The Pumping Block Assembly

    Use the 3-mm Allen wrench to unscrew and remove the 4 screws securing the pump block assembly to the instrument. Ion guide assembly Securing screws Pumping block Housing assembly Notice: To avoid damaging the ion guide assembly when removing the pumping block assembly from the instrument, do not grasp the ion guide by its metal lens plates.

  • Page 79: Removing The Differential Aperture Support And The Differential Aperture From The Ion Guide Assembly

    Notice: To avoid damaging the ion guide assembly when removing it from the differential aperture, do not grasp the ion guide by its metal lens plates. Instead, grasp the circuit boards on the top and bottom of the device. Pumping block assembly Ion guide assembly Differential aperture support and circuit...
  • Page 80 To remove the differential aperture support and the differential aperture from the ion guide assembly: Place the ion guide assembly with differential aperture on to a clean flat surface. Ion guide assembly Differential aperture support and circuit O-ring Differential aperture Connecting cables for differential aperture support...

  • Page 81: Cleaning The Differential Aperture

    To clean the differential aperture: Place the differential aperture in the glass vessel. Add Waters MS Cleaning Solution or 1:1 methanol/water to the vessel until the differential aperture is immersed completely. Place the vessel containing the differential aperture in the ultrasonic bath for 20 minutes.
  • Page 82 Suitable glass vessel in which to completely immerse the differential aperture when cleaning. • HPLC-grade deionized water. • Waters MS Cleaning Solution (part number 186006846) or HPLC-grade (or better) 1:1 methanol/water. • Holding container for used cleaning solution. • Large beaker.
  • Page 83 Notice: To avoid vibration-caused damage to the ion guide assembly, ensure that the bottom of the assembly is not in contact with the bottom of the glass vessel. Use the hook to carefully suspend the ion guide assembly in the glass vessel so that the bottom of the assembly does not touch the bottom of the vessel.

  • Page 84: Fitting The Differential Aperture And The Differential Aperture Support Onto The Ion Guide Assembly

    4.19.6 Fitting the differential aperture and the differential aperture support onto the ion guide assembly 4.19.6.1 Required materials • Clean, chemical-resistant, powder-free gloves. • 2.5-mm Allen wrench. • Flat-head screwdriver. • O-ring removal kit. Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.

  • Page 85: Fitting The Pumping Block Assembly And Ion Guide Assembly Onto The Instrument

    Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components. Notice: To avoid damaging the ion guide assembly when removing it from the pumping block assembly, do not grasp the ion guide by its metal lens plates.

  • Page 86: Maintaining The Esi Probe

    4.20 Maintaining the ESI probe Maintaining the ESI probe involves replacing the following components of the probe when required: Table 4–2: ESI probe consumables Component Name Part number ESI probe tip 700004207 Nickel gasket 700004208 PTFE liner tube 700000640 Ferrule 700000343 Conductive sleeve 700000969...
  • Page 87 To replace the ESI probe tip and gasket: Remove the ESI probe from the source (see page 39). Unscrew and remove the ESI probe tip by holding the probe shaft steady, using the 7-mm wrench, and unscrewing the probe tip using the 10-mm wrench, as shown in the following figure: 7-mm wrench 10-mm wrench...

  • Page 88: Replacing The Esi Probe Capillary

    Insert the new nickel gasket into the ESI probe tip so that the gasket is seated around the narrow, stainless steel tube inside the probe tip. Nickel gasket ESI probe tip Stainless steel tube Carefully slide the probe tip onto the ESI probe, ensuring that the capillary feeds through the stainless steel tube inside the probe tip.
  • Page 89 4.20.2.1 Required materials • Chemical-resistant, powder-free gloves • Combined 2.5-mm Allen wrench and cone extraction tool • 7-mm wrench • 8-mm wrench • 10-mm wrench • Needle-nose pliers • LC pump • HPLC-grade (or better) 1:1 acetonitrile/water • New capillary •...
  • Page 90 Tip: The end cover is secured by captive screws that you need not remove from the end cover of the probe. Simply loosen them to remove the end cover. Probe cable Captive screws End cover Rubber gasket Unscrew and remove the nebulizer adjuster knob. Probe cable Nebulizer adjuster knob ESI probe...
  • Page 91 Remove the nickel gasket from the probe tip. Nickel gasket Warning: To avoid spreading biohazardous, toxic, or corrosive contamination, dispose of the probe tip and nickel gasket in accordance with local environmental regulations. They can be contaminated with biohazards or toxic materials. Dispose of the nickel gasket in accordance with local environmental regulations.
  • Page 92 Unscrew and remove the knurled nut from the slide port, and remove the conductive liner tube from inside the knurled nut. Slide port Knurled nut PEEK union Conductive liner tube Important: Retain the knurled nut, which is required to reassemble the ESI probe.
  • Page 93 11. Unscrew and remove the PEEK union from the slide port using both the 7-mm and 8-mm wrenches. 8-mm wrench PEEK union 7-mm wrench Slide port Sample capillary Note: Hold the slide port steady by attaching the 7-mm wrench to the flattened grooves on the slide port’s collar, as shown in the figure.
  • Page 94 Slide the capillary into the slide port until the liner tube reaches the slide port’s narrow, threaded end, as shown in the following figure. Slide port Capillary Circular plate Lock nut Ferrule Liner tube Ensure that the slide port’s lock nut is screwed fully toward the slide port’s circular plate, as shown in the figure above.
  • Page 95 Insert the capillary and liner tube fully into the PEEK union, ensuring that the capillary end is flush with the liner tube end. Rationale: Doing so ensures that the capillary correctly aligns with the PEEK union. Slide port Liner tube Ferrule PEEK union Capillary...
  • Page 96 Screw the PEEK union onto the slide port, ensuring that the union is not fully tightened. Requirement: Ensure that you can still slide the capillary within the slide port and PEEK union. If the capillary is trapped and cannot move, loosen the PEEK union slightly.
  • Page 97 tube, and contacts the red PEEK tubing. This positioning is best achieved by pushing the capillary into place using the red PEEK tubing. Slide port Red PEEK tubing Probe inlet connector PEEK union Liner tube Sample capillary protruding through the liner tube to contact the red PEEK tubing Notice: To prevent damage to the ferrule, do not overtighten the PEEK...
  • Page 98 15. Slide the new conductive liner tube and knurled nut onto the capillary, and then tighten the knurled nut. Knurled nut Conductive liner tube Slide port PEEK union Probe inlet connector Red PEEK tubing Warning: To avoid eye injury from high-pressure liquid jet spray, wear safety goggles when performing the leak test.
  • Page 99 Notice: To avoid bending or damaging the protruding end of the capillary after you thread it through the ESI probe, take care when handling the probe assembly during the remainder of the procedure. 18. Carefully thread the capillary through the probe assembly until the slide port and PEEK union assembly reach the top of the probe assembly.
  • Page 100 20. Fit the nebulizer adjuster knob to the PEEK union, and fully tighten the knob. Probe cable Nebulizer adjuster knob ESI probe 21. Fit the end cover and gasket around the nebulizer adjuster knob. Important: Ensure that the end cover’s drip point is orientated so that, when viewed face-on, the probe’s warning label is directly to the left-hand side of the drip point, as shown in the figure below.

  • Page 101: Replacing The Ion Block Source Heater

    24. Carefully slide the probe tip onto the ESI probe, ensuring that the capillary feeds through the stainless steel tube inside the probe tip. 25. Screw the probe tip onto the ESI probe assembly. 26. Tighten the probe tip using the 7-mm wrench and the 10-mm wrench, as shown in the following figure: 7-mm wrench 10-mm wrench...
  • Page 102 To replace the ion block source heater: Remove the ion block assembly from the instrument (see page 68). Ensure that the isolation valve is closed. Isolation valve handle in closed Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the 2 captive screws securing the ion block cover plate.
  • Page 103 Use the combined 2.5-mm Allen wrench and cone extraction tool to loosen the captive PEEK terminal block securing screw. Heater cartridge assembly wires PEEK terminal block securing screw Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when removing the assembly and ceramic heater mounting block from the ion block.
  • Page 104 Use the needle-nose pliers to gently grasp the heat-shrink tubing on the heater cartridge assembly, and slide the assembly out of the ceramic heater mounting block Heat-shrink tubing Heater cartridge assembly Ceramic heater mounting block 10. Dispose of the heater cartridge assembly. Notice: To avoid damaging the heater cartridge assembly wires, do not bend or twist them when fitting the assembly to the ion block.

  • Page 105: Replacing The Source Assembly Seals

    4.22 Replacing the source assembly seals Warning: To avoid solvent vapors of biohazards and toxic materials leaking into the laboratory atmosphere, replace the seals listed below exactly as described in this section. Warning: To avoid solvent vapors of biohazards and toxic materials leaking into the laboratory atmosphere, replace the seals listed below at intervals not exceeding one year.
  • Page 106 • Nebulizer gas seal Probe adjuster nebulizer gas seal Probe adjuster assembly probe seal Use the O-ring removal kit to carefully remove the following seals from the source enclosure: • Source enclosure seal • Nebulizer gas seal • Desolvation gas seal Nebulizer gas seal Desolvation gas seal Source enclosure seal...

  • Page 107: Fitting The New Source Enclosure And Probe Adjuster Assembly Seals

    4.22.2 Fitting the new source enclosure and probe adjuster assembly seals 4.22.2.1 Required materials • Chemical-resistant, powder-free gloves • Wash bottle containing HPLC-grade (or better) 1:1 methanol/water • New seals Warning: To avoid personal contamination with biohazards or toxic materials, and to avoid spreading contamination to uncontaminated surfaces, wear clean, chemical-resistant, powder-free gloves when working with the source components.

  • Page 108: Replacing The Air Filter

    4.23 Replacing the air filter You must replace the air filter annually. 4.23.0.1 Required material New air filter To replace the air filter: Lift the instrument’s visor so that the source probe is fully exposed. Fully open the source enclosure. Disconnect the probe cable from the high-voltage connector, and leave the cable in a position that does not obstruct the air filter grill.

  • Page 109: Replacing The Instrument's Fuses

    Open the air filter grill by pulling the tab at the top of the grill toward you. Air filter grill Air filter grill TP03405 Remove and dispose of the old filter. Place the new filter flat on the inside part of the grill, with its edges beneath the metal lip.
  • Page 110 January 30, 2020, 715004599IVD Rev. C Page 110...

  • Page 111: A January 30, 2020Safety Advisories

    The following symbols warn of risks that can arise when you operate or maintain a Waters instrument or device or component of an instrument or device. When one of these symbols appear in a manual’s narrative sections or procedures, an accompanying statement identifies the applicable risk and explains how to avoid it.

  • Page 112: Specific Warnings

    A.1.1.1 Burst warning This warning applies to Waters instruments and devices fitted with nonmetallic tubing. Warning: To avoid injury from bursting, nonmetallic tubing, heed these precautions when working in the vicinity of such tubing when it is pressurized: •...

  • Page 113: Biohazard Warning

    To prevent ignition of flammable solvent vapors, Nitrogen gas must flow continuously through the mass spectrometer’s ion source when solvents are flowing into the source. The Xevo TQ-S micro IVD monitors the nitrogen gas input pressure, and MassLynx displays a nitrogen gas fail warning if the pressure falls below 400 kPa (4 bar, 58 psi).

  • Page 114: Notices

    A.1.1.5 Biohazard and chemical hazard warning These warnings apply to Waters instruments and devices that can process biohazards, corrosive materials, or toxic materials. Warning: To avoid personal contamination with biohazards, toxic materials, or corrosive materials, you must understand the hazards associated with their handling.

  • Page 115: Required Protection

    Use eye protection when refilling or replacing solvent bottles. Requirement: Wear clean, chemical-resistant, powder-free gloves when handling samples. A.5 Warnings that apply to all Waters instruments and devices When operating this device, follow standard quality-control procedures and the equipment guidelines in this section.
  • Page 116 注意︓規制機関か ら 明確な承認 を 受け ず に 本装置の変更や改造 を ⾏ う と 、 本装置の ユ ー ザ ー と し て の承認が無効 に な る 可能性が あ り ま す。 Warning: Use caution when working with any polymer tubing under pressure: •...
  • Page 117 Advertencia: se recomienda precaución cuando se trabaje con tubos de polímero sometidos a presión: • El usuario deberá protegerse siempre los ojos cuando trabaje cerca de tubos de polímero sometidos a presión. • Si hubiera alguna llama las proximidades. • No se debe trabajar con tubos que se hayan doblado o sometido a altas presiones.

  • Page 118: Warnings That Address The Replacing Of Fuses

    Vorsicht: Der Benutzer wird darauf aufmerksam gemacht, dass bei unsachgemäßer Verwendung des Gerätes die eingebauten Sicherheitseinrichtungen unter Umständen nicht ordnungsgemäß funktionieren. Attenzione: si rende noto all'utente che l'eventuale utilizzo dell'apparecchiatura secondo modalità non previste dal produttore può compromettere la protezione offerta dall'apparecchiatura. Advertencia: el usuario deberá...
  • Page 119 Advertencia: Para evitar incendios, sustituir los fusibles por aquellos del tipo y características impresos en los paneles adyacentes a las cubiertas de los fusibles del instrumento. 警告 ：為了避免火災，更換保險絲時，請使用與儀器保險絲蓋旁面板上所印刷之相同類型與 規格的保險絲。 警告：为了避免火灾，应更换与仪器保险丝盖旁边面板上印刷的类型和规格相同的保险丝。 화재의 위험을 막으려면 기기 퓨즈 커버에 가까운 패널에 인쇄된 것과 동일한 타입 경고...

  • Page 120: Electrical And Handling Symbols

    A.7 Electrical and handling symbols A.7.1 Electrical symbols The following electrical symbols and their associated statements can appear in instrument manuals and on an instrument’s front or rear panels. Symbol Description Electrical power on Electrical power off Standby Direct current Alternating current Alternating current (3 phase) Safety ground...
  • Page 121 Symbol Description Keep dry! Fragile! Use no hooks! Upper limit of temperature Lower limit of temperature Temperature limitation January 30, 2020, 715004599IVD Rev. C Page 121...
  • Page 122 January 30, 2020, 715004599IVD Rev. C Page 122...

  • Page 123: B External Connections

    B.1 External wiring and vacuum connections Rear panel connections appear in the figure below. Note that the connectors and controls not identified are for use by Waters engineers only. Figure B–1: Rear panel nano camera...

  • Page 124: Connecting The Oil-Filled Roughing Pump

    • PVC hose clamps (included in the Waters Rough Pump Connect Kit) • 1-inch ID vacuum hose (included in the Waters Rough Pump Connect Kit) Bear in mind these requirements when connecting the roughing pump: • The pump must be horizontal, or within one degree of horizontal.
  • Page 125 For proper ventilation, the following minimum clearances must apply: Left-side minimum clearance is Back-side minimum clearance 15.24 cm (6 inches) is 15.24 cm (6 inches) Right-side minimum clearance Front-side minimum clearance is 15.24 cm (6 inches) is 35.56 cm (14 inches) To connect the roughing pump: Warning: To avoid personal contamination with biohazards...
  • Page 126 Using the NW25 center rings and clamp, and the 7-mm nut driver, attach the flanged end of a length of 1-inch ID vacuum hose to the NW25 tee as shown above, and attach the 10-mm reducer fitting and a length of 10-mm ID nylon tubing to the middle (perpendicular) port on the tee.

  • Page 127: Connecting Electric Cables To The Oil-Filled Roughing Pump

    B.2.1 Connecting electric cables to the oil-filled roughing pump Figure B–2: Roughing pump electrical connections: Instrument rear panel To power source Roughing pump d.c. connector To make the electrical connections to the roughing pump: Connect the relay cable from the roughing pump d.c. connector to the pump connector on the instrument’s rear panel.

  • Page 128: Connecting The Edwards Oil-Free Roughing Pump

    10-mm reducer fitting (included in the installation kit) • 10-mm ID nylon tube (included in installation kit) • 12.7-mm clear PVC exhaust tubing (included in the Waters Rotary Pump Kit) • PVC hose clamps (included in the Waters Rotary Pump Kit) •...
  • Page 129 • The ambient temperature of the area in which you place the pump must range between 15 and 40 ºC. • The pump must be oriented so that it permits easy access to the gas ballast valve. For proper ventilation, the following minimum clearances must apply: Back-side minimum clearance Right-side minimum clearance is 15.24 cm (6 inches)

  • Page 130: Connecting Electric Cables To The Edwards -Free Roughing Pump

    Connect the opposite end of the 10-mm nylon tube to the 10-mm source vent port on the instrument’s rear panel. Notice: To prevent serious damage to the instrument, two separate exhaust systems are required: one for nitrogen, the other for the roughing pump.

  • Page 131: Connecting To The Nitrogen Gas Supply

    • Sharp knife • Wrench • 6-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) • Nitrogen regulator To connect the nitrogen gas supply: Use the sharp knife to cut the 6-mm PTFE tubing into two lengths; one approximately 5 m long, the other approximately 4.5 cm.

  • Page 132: Connecting To The Collision Cell Gas Supply

    Figure B–4: Gas and exhaust connections Source vacuum Source vent Collision cell gas inlet (Argon) Turbo vacuum Nitrogen inlet Connect the free end of the 4.5-cm length of PTFE tubing to the nitrogen supply in-line filter Connect one end of the 5-m length of PTFE tubing to the free port on the nitrogen supply in-line filter.

  • Page 133: Connecting The Nitrogen Exhaust Line

    B.6.0.1 Required materials • Chemical-resistant, powder-free gloves • Sharp knife • 12-mm PTFE tubing (included in the Waters Rough Pump Connect Kit) ® • snoop (or equivalent) leak detector liquid To connect the nitrogen exhaust line: Warning: To avoid exposure to biohazards or toxic LC solvents carried in the nitrogen exhaust, ensure that the nitrogen exhaust is vented via the nitrogen exhaust trap bottle and laboratory exhaust system.
  • Page 134 Requirement: Cut the tubing squarely (that is, perpendicularly to its horizontal axis). Connect one end of the tubing to the exhaust port on the rear panel and the other end to one of two ports on the exhaust trap bottle. Cut a length of 12-mm tubing long enough to connect the exhaust trap bottle to the exhaust vent.

  • Page 135: Connecting The Liquid Waste Line

    B.7 Connecting the liquid waste line B.7.0.1 Required material Chemical-resistant, powder-free gloves To connect the liquid waste line: Warning: To prevent personal contamination with biohazards or toxic materials, always wear chemical-resistant, powder-free gloves while performing this procedure. The waste line and connection can be contaminated.

  • Page 136: Connecting The Workstation

    Requirement: To ensure optimum performance and signal integrity, use only Waters-supplied, shielded, CAT 5 ethernet cables. To connect the workstation: Connect the monitor to the PC. Connect one end of a shielded network cable to the appropriate port on the rear panel of the instrument.

  • Page 137: I/O Signal Connectors

    Connect the free end of the shielded network cable to the port labeled “Instrument LAN” on the workstation’s rear panel. To connect the workstation to the power source: Select the correct power cord for your location. Connect the female end of the power cord to the power port on the rear panel of the workstation.

  • Page 138: Signal Connections

    B.9.1 Signal connections Table B–1: Instrument analog-out/event-in connections Signal connections Description Analog (Out) Used for analog chart output functionality. The output voltage range is 0 to 1 V. The resolution of the voltage output is 12 bits. Stop Flow (Out) Used to stop the solvent flow if the nitrogen gas supply fails.
  • Page 139 Insert the clamp and shield (with the bend facing down) into the connection cover, and loosely tighten with one self-tapping screw. Clamp Connection cover Shield TP02586 Insert the connector with the signal cable into the connection cover, and position the clamp over the cable leads. Tighten the clamp into place with the second self-tapping screw.

  • Page 140: Connecting To The Power Supply

    States and HAR-type (or better) in Europe. The main power cord must be replaced with one of adequate rating. For information regarding what cord to use in other countries, contact your local Waters distributor. Connect the female end of the power cord to the receptacle on the rear panel of the instrument.

  • Page 141: C January 30, 2020Materials Of Construction And Compliant Solvents

    C.1 Preventing contamination For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). Visit https://www.waters.com. C.2 Items exposed to solvent The items that appear in the following table can be exposed to solvent. You must evaluate the safety issues if the solvents used in your application differ from the solvents normally used with these items.

  • Page 142: Solvents Used To Prepare Mobile Phases

    Table C–1: Items exposed to solvent (continued) Item Material Probe shaft PEEK Push-in gas fittings Nickel/brass Solvent waste/leak management Tygon tubing Source enclosure Alochromed aluminum Source enclosure view port Toughened plate glass Drip trays Aluminium Trap bottle Polypropylene Trap bottle push-in fittings Nitrile butadiene rubber, stainless steel, polybutylene terephthalate, and polyoxymethylene...

  • Page 143: D Plumbing The Intellistart Fluidics System

    For information on preventing contamination, refer to Controlling Contamination in LC/MS Systems (part number 715001307). You can find this document on https://www.waters.com; click Services and Support > Support. D.2 The selector valve The selector valve is located on the right-hand side of the instrument, behind the visor.

  • Page 144: Plumbing Schematic

    Table D–1: Selector valve connections: (continued) Port Component Input/Output LC column Input Xevo TQ-S micro IVD ion source Output D.3 Plumbing schematic Selector valve Waste column Xevo Pump Wash TQ-S micro IVD probe Reservoir A Reservoir B Requirement: Ensure that the end of the tubing is fully submerged in the solvent in the wash reservoir.
  • Page 145 S, W, R, A and B F130 The tubing for the sample reservoirs (ports A and B) is not Important: user-serviceable. To replace the tubing, contact Waters to arrange an engineer visit. January 30, 2020, 715004599IVD Rev. C Page 145...
  • Page 146 January 30, 2020, 715004599IVD Rev. C Page 146...

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