Abbott CELL-DYN 3200 System Operator's Manual page 59

Section 1
Syringe Assembly
Sample Transfer Peristaltic Pump
HGB Flow Cell and Mixing Chamber
WBC Mixing Chamber
RBC/PLT Mixing Chamber
Wash Block
CELL-DYN ® 3200 System Operator's Manual
9140181H—October 2001
There are two syringe driver assemblies, each containing two syringes. Each
syringe is operated by its own stepper motor. The function of each syringe is
described below:
• Diluent/Sheath Syringe — (1) delivers a specific volume of diluent to
transport the RBC segment from the Shear Valve to the RBC/PLT Mixing
Chamber and to dilute the segment prior to measurement, and (2) delivers a
specific volume of diluent to transport the HGB segment from the Shear
Valve to the HGB Mixing Chamber and to dilute the segment prior to
measurement.
• HGB Lyse Syringe — delivers a specific volume of HGB Lyse to the HGB
Mixing Chamber/Flow Cell to further dilute the HGB segment prior to
measurement.
• WBC Lyse Syringe — delivers a specific volume of WBC Lyse to transport
the WBC segment from the Shear Valve to the WBC Mixing Chamber and to
dilute the segment prior to measurement.
• Sample Injection Syringe — injects a specific volume of the diluted sample
into the Optical Flow Cell for RBC/PLT, WBC (WOC), and WBC (NOC)
measurements.
The Sample Transfer Peristaltic Pump is composed of a rotor and a pump tube
holder. It is used to transfer the WBC dilution, RBC/PLT dilution, and HGB/NOC
dilution to the Optical Flow Cell from their respective mixing chambers.
The HGB Flow Cell Assembly is integrated with a mixing chamber and contains
the following components:
• A fully enclosed (light-tight) mixing chamber with optical windows and
electronics
• An LED Light Source
• A Photodetector for measuring the light transmitted.
The WBC Mixing Chamber is used to swirl mix the WBC dilution.
The RBC/PLT Mixing Chamber is used to swirl mix the RBC/PLT dilution.
There are two Wash Blocks on the instrument, one for the Open Sample Aspiration
Probe and one for the Closed Sample Needle. Both Wash Blocks work in a similar
fashion: After aspiration, they move down the outside of the probe or needle,
rinsing it with diluent. The diluent rinse is then transferred to the waste chamber.
Use or Function
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