Abbott CELL-DYN 3200 System Operator's Manual page 139

Section 4
Measurement Specifications — CELL-DYN 3200SL and CS
Measurement Channels
WBC (WOC)
WBC (NOC)
RBCs and PLTs
HGB
CELL-DYN ® 3200 System Operator's Manual
9140181H—October 2001
Laser optics for WBC and RBC/PLT
Hemoglobin Absorbance
Reticulocyte %
Method Laser light scatter
Light source Vertically polarized 5–10 mW helium-neon laser
Wave length 632.8 nm
Dilution 1:50 of blood in WBC lyse
Data Collection Four angles measured: 0°, 10°, 90°, and 90° depolarized. Data
collected in 256 channels for each angle of light scatter
Method Laser light scatter
Light source Vertically polarized 5–10 mW helium-neon laser
Wave length 632.8 nm
Dilution 1:218 of blood in diluent/sheath and HGB Lyse
Data Collection Four angles measured: 0°, 10°, 90°, and 90° depolarized. Data
collected in 256 channels for each angle of light scatter
Method Laser light scatter
Light source Vertically polarized 5–10 mW helium-neon laser
Wave length 632.8 nm
Dilution 1:1,675 of blood in diluent/sheath
Data Collection Three angles measured: 0°, 10°, and 90°. Data collected in 256
channels for each angle of light scatter
Method Modified methemoglobin
Light Source LED, wavelength: 555 nm
Wavelength 555 nm
Dilution 1:218 of blood in diluent/sheath and HGB Lyse
Data Collection Five absorbance readings for the blank, lowest and highest
readings eliminated. The three remaining absorbance readings
are averaged for the HGB reference. Five absorbance readings
for the sample, lowest and highest readings eliminated. The
three remaining absorbance readings are averaged for the
HGB sample.
Performance Characteristics and Specifications
4-7