Abbott CELL-DYN 3200 System Operator's Manual page 114

Principles of Operation
Flow Cytometry
RBC Parameters
Figure 3.9 RBC Data and Histogram
RBC Count
MCV
HCT
3-20
All numeric and frequency size distribution data are automatically displayed on the
RUN screen in the format selected. The size distribution data for the red cells is
displayed graphically as a histogram using 0° data. The size distribution data is
plotted on the X axis. The relative number of cells is normalized and plotted on the
Y axis. The RBC data are shown in Figure 3.9.
The Red Blood Cell Count is directly measured, and is expressed as follows:
RBC = # x M/µL
Counts below 1.0 x M µL are displayed to three decimal places. The RBC count is
corrected for coincidence and WBC interference.
The Mean Cell Volume is the average volume of the individual red blood cells.
The MCV is derived from the RBC size distribution data on the 0°, 10°, and 90°
histograms, and is expressed in femtoliters.
The Hematocrit is the ratio of red blood cells to plasma and is expressed as a
percentage of the whole blood volume. The HCT is calculated from the red blood
cell count and the mean cell volume as follows:
HCT = (RBC x MCV)/10
CELL-DYN ® 3200 System Operator's Manual
9140181H—October 2001
Section 3