Abbott CELL-DYN 3200 System Operator's Manual page 307

Section 6
Pre-Calibration Check List
CELL-DYN ® 3200 System Operator's Manual
9140181H—October 2001
Sample Age
• All blood samples should be less than four hours old when calibration
begins. These samples must be less than eight hours old by the time
1
calibration is completed.
• No more than two hours should elapse between the CELL-DYN 3200 run and
the assay by reference methodology or reference instrument. If samples are
run on the CELL-DYN 3200 first, assay by reference methodology should be
completed within one hour. (Certain reference methodologies are sensitive to
RBC swelling caused by in vitro deoxygenation.)
Sample Amount
The whole blood sample volume should be at least 12 mL to accomplish the
following:
• Obtain reference values on a reference instrument prior to calibration.
• Calibrate both the Open and Closed Modes.
• Confirm calibration of both modes.
NOTE: Because only one fresh whole blood sample is used in Auto-Cal, it
is important that a representative sample be selected to calibrate the
instrument. A sample containing abnormalities may adversely
affect calibration.
Number of Cycles
• Samples should be assayed first on a reference instrument or by reference
methodology and then on the CELL-DYN 3200.
• Auto-Cal Method: A single fresh whole blood sample should be cycled for
10 consecutive runs when calibrating in either Open or Closed Mode.
• Enter Factor Method: Five fresh whole blood samples should be cycled twice
each for a total of at least ten runs when calibrating in either Open or Closed
Mode. Additional samples and/or repetitions of the specimens may be used
to achieve calibration accuracy beyond NCCLS recommendations.
Follow the procedures outlined in the Pre-Calibration Check List to ensure the
instrument is ready for calibration. Use the Problem List to note any problems
encountered. Make copies of both lists as needed.
NOTE: Always complete the Pre-Calibration procedures before beginning any
calibration.
Calibration Procedures
6-9