Abbott CELL-DYN 3200 System Operator's Manual page 341

Section 6
Calibration Procedure — Mode to Mode
Determining the Closed Mode Mean
CELL-DYN ® 3200 System Operator's Manual
9140181H—October 2001
Follow the procedure below for calibrating the instrument in the Closed Mode.
NOTE: Use the same Calibrator or Fresh Whole Blood samples for calibrating
Open and Closed Modes.
1. If necessary, go to the RUN menu and press [CHANGE SAMPLER] to select the
Closed Mode.
2. In the MAIN MENU, press [CALIBRATION] to display the main CALIBRATION
menu.
3. Obtain a printout of the current Closed Sampler Calibration Factors as
follows:
a. If necessary, press [CLOSED SAMPLER] to display the Closed Sampler
Calibration Factors.
b. Press [PRINT] to obtain a printout of these factors. Save this printout. It
will be used when completing Worksheet 6 at the end of this section.
4. Open an empty QC file as follows:
a. In the MAIN MENU, press [RUN] to display the RUN menu.
b. If necessary, press [CHANGE SAMPLER] to select Closed Mode.
c. Press [SPECIMEN TYPE] to display the SPECIMEN TYPE menu.
d. Use the arrow keys on the keyboard to move the cursor to an empty QC
file and type the desired name in the <File Name> field.
e. Press Enter to save the name. Use the Up Arrow key to move the cursor
back to the file name.
Press [QC SPECIMEN] to return to the RUN screen.
f.
WARNING: Potential Biohazard. Consider all specimens, calibrators,
and controls that contain human blood as potentially infectious. Wear
gloves, lab coats, and safety glasses and follow other biosafety procedures
as specified in the OSHA Bloodborne Pathogen Rule (29 CFR 1910.1030)
or other equivalent biosafety procedures.
5. Calibrating the CS model (Calibrator or Fresh Whole Blood):
a. Mix the specimen well. If calibrator material is used, follow the mixing
instructions found in the package insert. If fresh whole blood is used, mix
it well by inverting the tube at least ten times. Do not shake the specimen.
b. Place the well-mixed specimen in the Tube Retainer, close the door, and
press the Touch Plate.
c. If calibrator material is used, repeat this process until a minimum of 6
runs has been completed.
d. If fresh whole blood is used, repeat this process until a minimum of 10
runs has been completed.
e. Go to step 7.
Calibration Procedures
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