Waters 2690 Quick Start Manual
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Waters 2690 Quick Start Manual

Separations module
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Waters 2690
Separations Module
Quick Start Guide
34 Maple Street
Milford, MA 01757
WAT553-03TP, Revision 3

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Summary of Contents for Waters 2690

  • Page 1 Waters 2690 Separations Module Quick Start Guide 34 Maple Street Milford, MA 01757 WAT553-03TP, Revision 3...
  • Page 2 This manual is believed to be complete and accurate at the time of publication. In no event shall Waters Corporation be liable for incidental or consequential damages in connection with, or arising from, the use of this manual.
  • Page 3 Quick Summary The figure below summarizes the information contained in this Quick Start Guide. Use it as a reference after you have read the entire document. Start Set Flow Rate and Adjust the Composition Seal Pack Power on the Separations Module Set Sparge Rate Load the...
  • Page 4 Conventions Used in This Guide This Quick Start Guide uses the following conventions: • Bold text indicates user input or action. For example, press Enter. • When you are instructed to “press the key”, press the indicated keypad key. • When you are instructed to “press the screen key,”...
  • Page 5 What Is In This Guide? The Waters 2690 Separations Module Quick Start Guide contains basic procedural information to help you set up the Waters 2690 Separations Module and make a run. In addition, this guide contains detailed flow charts that provide Waters-recommended steps to prime, equilibrate, and purge both degasser and sparge-based 2690 Separations Modules.
  • Page 6 • 2690 XE – Provides the capabilities of the 2690 base product, replaces the helium sparge system with a four-channel in-line vacuum degasser, and adds a column heater and a sample heater/cooler.
  • Page 7 Module for Operation Before you can prepare the Separations Module for operation, you need to power on the 2690 Separations Module by moving the power switch (located at the top of the left side panel) to the (On) position. The startup diagnostics routine begins.
  • Page 8 (Figure 2-2) while the diagnostics are running. If the startup diagnostics routine is not successful, refer to the Waters 2690 Separations Module Operator’s Guide , Section 8.5, Troubleshooting. When the startup diagnostics routine is complete, the Separations Module enters the Idle mode.
  • Page 9 The Separations Module controls other non-IEEE devices in the HPLC system using the I/O connections on the rear panel. Refer to the Waters 2690 Separations Module Operator’s Guide , Section 2.7.1, I/O Signal Connections, for the procedure to make I/O connections.
  • Page 10 Press the screen key to return to the Configuration screen. 5. Press Exit. The Separations Module is ready to control Waters detectors using separation methods, sample sets, or sample templates. Controlled by Millennium Mode In this remote-control mode, a Millennium Chromatography Manager workstation running...
  • Page 11 Controlled by MassLynx Mode In this remote-control mode, Micromass MassLynx NT software (Version 3.1 Build 004 or higher) controls the 2690 Separations Module. MassLynx software is used with Micromass mass spectrometry (MS) detectors, using the IEEE-488 interface between the Micromass computer and the 2690 Separations Module.
  • Page 12 Refer to the Waters 2690 Separations Module Operator’s Guide , Section 5.2, Making Automatic Runs Under Millennium Control, for details on making runs under Millennium control. Controlled by Operate Gradient by Event In In the Operate Gradient by Event In mode, an external autosampler (a Waters 2700 Sample Manager, for example) signals the Solvent Management System of the Separations Module to begin a gradient.
  • Page 13 Controlled via RS-232 (ASCII) or (Binary) Modes In these remote-control modes, a non-Waters data system (a mass spectrometry system, for example) controls the Separations Module using RS-232 ASCII or binary communications. The Separations Module disconnects from the IEEE-488 interface bus when either of these modes is selected.
  • Page 14 Figure 2-4 Report Options Dialog Box 2. Select the destination for the report in the drop-down list. 3. Select any option and press any numeric key to enable or disable the option, then press Enter. An “X” in the box means the option is enabled. The options include: •...
  • Page 15 • No changes sent to the Separations Module from a remotely connected Millennium Chromatography Manager workstation, MassLynx computer, or external autosampler • An error condition that suspends the operation of the Separations Module You can leave the Separations Module in the shutdown state indefinitely. The separation method you specify in the Auto Shutdown dialog box defines the initial conditions that are applied after the specified period of inactivity.
  • Page 16 If you want to use Auto Shutdown to turn off the lamp of a 2487 or 486 detector, program a Lamp Off event and specify a time of INIT in the I/O Events Timed Table screen (see Section 6.2.5, Setting I/O Parameter Values in the Waters 2690 Separations Module Operator’s Guide).
  • Page 17 For optimal performance of your Separations Module, perform the steps identified in Figure 2-6. Note: For details on how to prepare both degasser and sparge-based 2690 Separations Modules for operation, refer to Chapter 6, Recommended Preparation Procedures Start Prime the Solvent...
  • Page 18 Note: Do not run the degasser for extended periods of time without analytical solvent flow. Doing so causes solvent vaporization that results in recondensation (once dissolved gasses have been removed), possibly leading to a misdiagnosis of a vacuum chamber leak. Note: For proper operation of the in-line vacuum degasser, you must fill each vacuum chamber with solvent.
  • Page 19 Sparging You set the initial and maintenance sparge cycles from the Sparge screen. You can set different sparge cycles for each of the four solvent reservoirs. To program the sparge cycles from the Direct Function menu: 1. Press the Direct Function screen key in the Status screen.
  • Page 20 To perform a dry prime: 1. Set up the reservoirs as described in the Waters 2690 Separations Module Operator’s Guide . Section 2.6, Making Fluidic Connections. 2. Insert the solvent tubing into the appropriate reservoir(s). Be sure the detector waste line and the sample loop waste line drain into an appropriate container.
  • Page 21 10. Press a screen key corresponding to the solvent line with which you want to prime the system. Waters recommends that you prime using the solvent with the lowest viscosity to help purge air from the lines, especially if the in-line vacuum degasser is installed.
  • Page 22 100% for the first solvent you want to use for wet priming. Waters recommends that you start the wet prime using the solvent with the lowest viscosity to help purge air from the lines, especially if the in-line vacuum degasser is installed.
  • Page 23 Diag screen key in the Main screen. The Diagnostics screen appears (Figure 2-12). Refer to the Waters 2690 Separations Module Operator’s Guide , Section 8.3, Performing Main Diagnostics, for more information on the Diagnostics screen. Preparing the Separations Module for Operation...
  • Page 24 F98SM7885M G98SM4582M Figure 2-12 Diagnostics Screen 2. Attach the syringe adapter to the syringe (both are found in the Startup Kit). 3. Remove the inlet filter from the seal wash inlet tube. 4. Fill the syringe with the seal wash solution, then attach the syringe adapter to the end of the seal wash inlet tube.
  • Page 25 2.4 Preparing the Sample Management System Figure 2-13 shows the steps in preparing the sample management system for operation. Start Purge the Sample Adjust the Management Seal Pack System Load the Prime the Needle Carousels Wash Pump Separations Module is Ready to Operate Figure 2-13 Preparing the Sample Management System for Operation 2.4.1 Purging the Sample Management System...
  • Page 26 4. Select Purge Injector, then press Enter. The Purge Injector dialog box appears (Figure 2-14). Figure 2-14 Purge Injector Dialog Box 5. Enter the number of sample loop volumes in the Sample Loop Volumes field (use the default value of 6.0 volumes), then press Enter. The solvent management system ramps up to a predetermined flow rate to flush the sample loop with the selected volume.
  • Page 27 Ion exchange To prime the needle-wash pump: 1. Be sure the needle-wash fluidic lines are properly installed, as described in the Waters 2690 Separations Module Operator’s Guide , Section 2.6.6, Installing Needle Wash and Plunger Seal Wash Reservoirs. 2. Press the Diag screen key in the Main screen.
  • Page 28 • You change the seal pack • You rebuild the seal pack Before you adjust the seal pack, purge the sample management system (Section 2.4.1, Purging the Sample Management System) to ensure that there is no air in the syringe. Air in the system may cause Alarm Seal Geometry or Alarm Missing Restrictor dialog boxes to erroneously appear.
  • Page 29 Table 2-2 Carousel Codes and Colors (Continued) Code Color Vial Numbers Green 73 to 96 White 97 to 120 To load the carousels: 1. Open the carousel door. The Door is Open dialog box appears in the screen (Figure 2-15). Figure 2-15 Door is Open Dialog Box 2.
  • Page 30 Performing a Direct Control This section describes the procedures for performing a direct control (manual) run. Figure 3-1 provides an overview of the procedures. To make an automatic run, refer to Section 4, Making Automatic Runs. Menu Press Status Select Separation Select the Direct Method, or Enter Function to Perform...
  • Page 31 Figure 3-2 First Page of Status Screen Press the Next Page screen key to display the second page of the Status screen (Figure 3-3). Figure 3-3 Second Page of Status Screen Performing a Direct Control Run...
  • Page 32 V1 – V4 Indicates the position (open or closed) of each of the valves in the sample management system. See the Waters 2690 Separations Module Operator’s Guide , Section 1.3, Sample Management System Overview, for a description of the valves.
  • Page 33 Table 3-1 Status Screen Parameters (Continued) Parameter Description Indicates the current position of the syringe. Valid positions are: • Home – Default position • Empty – Highest position (to purge the syringe) • Full – Lowest position (to draw in sample) Vial Indicates the number of the vial that is currently under the injector.
  • Page 34 External Temp 1 • Col 2 • External Temp 2 Enabled if the optional column heater module is configured with the Waters 2410 or 410 detector. To access the direct control functions: 1. Press the Menu/Status key (if necessary) to display the Status screen (Figure 3-2).
  • Page 35 Removes mobile phase from the sample loop and from the syringe and fills it with fresh mobile phase. As an option, performs a compression test. Purge 2410/410 Reference Purges the Waters 2410 or 410 RI detector reference (if applicable) cell. Program Sparge (if Sets the sparge rate for each reservoir.
  • Page 36 • Controlled by MassLynx mode System Controller or No Interaction Mode If your HPLC system uses the 2690 Separations Module in a System Controller or No Interaction mode, you make an automatic run using a sample set or sample template stored in the Separations Module.
  • Page 37 4.1 Making Automatic Runs in Stand-Alone Mode Figure 4-1 shows the steps for setting up and making an automatic run with a Separations Module in a stand-alone configuration (System Controller or No Interaction mode). Press the Samples Screen Key on the Main Screen Enter Information Select the Separation Required by the...
  • Page 38 Running a Sample Set Note: If you plan on importing sample sets stored on a diskette, refer to the “Importing Methods” discussion at the end of this section. To run a sample set in a standalone mode: 1. Press the Run Samples screen key in the Main screen (Figure...
  • Page 39 • Initial Conds – Starts the solvent management system at the initial conditions (specified in the first line of the sample set) and begins equilibrating the temperature in the sample heater/cooler and/or column heater. When the system is equilibrated, press the screen key.
  • Page 40 You can perform an automated run in a stand-alone mode using a sample template stored in the Separations Module. Screen prompts give you step-by-step directions to prepare for the run. Refer to the Waters 2690 Separations Module Operator’s Guide , Chapter 6, Creating Methods, Sample Sets, and Sample Templates, for a description of sample templates.
  • Page 41 Figure 4-5 Setup Dialog Box 3. Enter the number of samples to run, then press the Continue screen key. 4. Enter the location of the first sample vial, then press the Continue screen key. You must use consecutive sample vials on one or more carousels. 5.
  • Page 42 You must save the changes to the modified separation method before you can apply the method to all subsequent injections that use that separation method. Use the procedures in the Waters 2690 Separations Module Operator’s Guide , Section 6.1, Creating and Editing Separation Methods, to modify and save the separation method.
  • Page 43 STOP complete; the Separations Module cannot detect a partially imported method. Waters recommends that you carefully review any impor ted method before you run the method. An error message appears if you attempt to import a method that already exists in the Separations Module.
  • Page 44 Figure 4-7 Methods Screen 3. Press the Export Methods screen key. The Export Methods screen appears, displaying a list of currently stored methods (Figure 4-8). Figure 4-8 Export Methods Screen 4. Select the method you want to export to the disk, then press Select Method.
  • Page 45 1. Create a chromatographic system that includes the Waters 2690 Separations Module: • For Millennium software version 2.1x, create a system that includes the 2690 solvent management system and the sample management system. Refer to the Millennium documentation for information on creating a chromatographic system.
  • Page 46 4. From Millennium³² software, run the samples in Run Samples (QuickSet in Millennium versions earlier than 3.20) using the method set you created in step 3 above. When you enter Run Samples, use the HPLC system you created in step 1, above. Refer to the Millennium documentation for information on using Run Samples.
  • Page 47 4.4 Stopping a Run To stop a run in progress, press one of the following keys from the Status screen: • Stop Inject (screen key) – Stops the current injection and displays a dialog box requesting you to abort or resume the injection •...
  • Page 48 Powering Off the Separations Module Before Powering Off Before you power off the Separations Module, remove any buffered mobile phase present in the fluidic path. Attention: To avoid damaging your column, remove the column before you perform the STOP following procedure. To remove buffered mobile phase from the fluidic path of the Separations Module: 1.
  • Page 49 Separations Module, refer to the Waters 2690 Separations Module Operator’s Guide , Chapter 4, Section 4.4, Performing Direct Functions. Note: All flow chart references in this chapter refer to sections or figures in the Waters 2690 Separations Module Operator’s Guide. Recommended Preparation Procedures...
  • Page 50 Figure 6-1 outlines how to prime, equilibrate, and purge a sparge-based Separations Module that is new or dry. Note: All flow chart references refer to sections in the Waters 2690 Separations Module Operator’s Guide. Start Fill the Solvent Reservoirs with...
  • Page 51 Injector” in Section 4.4.2) To maximize degasser efficiency (and fill all four degasser chambers with solvent), Waters recommends that all four solvent reservoirs be filled (even if four solvents are not used). If you choose water as one of the “unused”...
  • Page 52 Separations Module that has been powered off or is idle with no solvent flow. Note: All flow chart references refer to sections in the Waters 2690 Separations Module Operator’s Guide. Start...
  • Page 53 Separations Module that has been powered off or is idle with no solvent flow. Note: All flow chart references refer to sections in the Waters 2690 Separations Module Operator’s Guide. Start...
  • Page 54 Separations Module. Note: All flow chart references refer to sections or figures in the Waters 2690 Separations Module Operator’s Guide. Start Remove the Solvent...
  • Page 55 Separations Module. Note: All flow chart references refer to sections or figures in the Waters 2690 Separations Module Operator’s Guide. Start Remove the Solvent...

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