Waters Alliance e2695 Operator's Manual page 139

7.6.2.2 Install a new syringe
If you need to install a syringe different in size from the original syringe, refer to the following table
for available syringe sizes. Be sure to enter the new syringe size in the Configuration screen. (See
page 140.)
Notice: It is very important not to bottom the plunger in the syringe barrel, because this will
damage the Teflon plunger tip and negatively affect syringe lifetime.
Table 7–3: Syringe sizes
Standard size
250-µL
To connect and initialize the syringe:
1. The syringe package contains the unassembled plunger and spare syringe barrel. Remove
each component from the package and wet the Teflon tip of the plunger with mobile phase
or methanol.
2. Gently insert the plunger into the syringe barrel, about 3/4s down the length of the barrel.
3. Dip the end of the syringe into mobile phase or MeOH, and draw the liquid until the syringe
is partially filled.
4. Hold the syringe vertically and gently tap the barrel so that the liquid settles on the plunger
tip and any air rises to the top.
5. Gently push the plunger to expel the air, without bottoming the plunger in the syringe barrel.
6. Screw the new syringe partially into the mounting bracket. Do not tighten.
Figure 7–12: Syringe and mounting bracket
Optional sizes
25-µL, 100-µL, 2500-µL
TP0136
February 27, 2018, 715003794 Rev. C
Page 139