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2.
On the Status screen, press Direct Function.
3.
Select Wet Prime, and then press Enter.
4.
Gently shake the filters in the reservoirs to remove any bubbles.
5.
In the Wet Prime dialog box, specify the flow rate and duration of the priming (using the
composition entered in the Status screen), and then press the OK screen key.
Result:
The solvent management system begins to operate at the end of the specified time
period, the solvent management system returns to the previous conditions, and the
separations module enters the idle mode.
6.
Repeat
step 1
4.4.3.1
Equilibrate solvents in the in-line vacuum degasser
To equilibrate solvents in the vacuum degasser after you wet primed the system:
1.
In the Status screen, specify the initial solvent composition for the run.
2.
Set the degasser mode parameter to ON.
3.
Press the Direct Function screen key.
4.
Select Wet Prime, and then press Enter.
5.
Enter a flow rate of 0.000 mL/min and a time of 5 min.
After the solvents in the vacuum degasser are equilibrated and the separations module is
prepared to run, perform any of the following functions as necessary:
•
Purge the injector
•
Purge the refractometer reference cell
•
Condition the column
•
Equilibrate the system
4.4.3.2
Purge the injector
Purge the injector for these reasons:
•
To remove any traces of solvent from a previous run
•
To clear air bubbles from the flow path or the syringe
•
To switch to a new (miscible) solvent after you wet prime
The purge process can include a compression check to test the needle seals.
Notice:
To avoid precipitating salts in the separations module, use an intermediate solvent
such as water when you change from buffers to solvents of high organic content. See
"Solvent miscibility" on page 228
To purge the injector:
1.
Press the Direct Function screen key in the Status screen.
2.
Select the Purge Injector option, and then press Enter.
3.
In the Purge Injector dialog box, specify 6 sample loop volumes.
through
step 5
for each solvent, as appropriate.
for information on solvent miscibility.
February 27, 2018, 715003794 Rev. C
Page 77
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Troubleshooting
