Overview
Calibration reference files and DRE file consist of two columns of numbers
separated by any number of spaces or characters. The first column contains
the reference peak masses (m/z), and the second column contains the
reference peak intensities.
Most samples can be purchased from the Sigma chemical company. To order
them, contact Sigma at http://www.sigma.sial.com. This site includes a list of
worldwide Sigma offices, many with local toll-free numbers.
FC-70 can be purchased from Fluorochem at http://www.fluorochem.net.
Editing a calibration reference file
Calibration reference files (*.ref) are stored in the MassLynx/Ref folder.
Calibration reference files are created or edited using any Windows® text
editor like Notepad.
To save the reference file after editing, click File > Save to save the file under
the current name, or click File > Save As to save as a new reference file with a
new name.
Textual information or comments can be stored in the reference file. Lines
that are textual information or comments must start with the semicolon (;)
character.
Calibration reference file
Heptacosa
Use the heptacos.ref file for calibration in EI+.
Heptacos.ref file:
Calculated
Intensity
m/z value
49.99680
0.73
68.99518
100.00
92.99518
0.56
C-2
Calibration Reference Information
Calculated
Intensity
m/z value
130.99199
36.31
149.99039
1.29
163.99350
0.56
Calculated
Intensity
m/z value
213.99030
0.76
218.98560
38.07
225.99030
0.56