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[ CARE AND USE MANUAL ]
Atlantis Premier BEH C
CONTENTS
I.
INTRODUCTION
II.
GETTING STARTED
a. eCord Installation
b. Column Installation
c. Column Equilibration
d. Initial Column Efficiency Determination
III.
COLUMN USE
a. Sample Preparation
b. pH Range
c. Solvents
d. Pressure
e. Temperature
f. Replacing the VanGuard FIT Cartridge
IV.
COLUMN CLEANING, REGENERATION,
AND STORAGE
a. Cleaning and Regeneration
b. Storage
V.
eCORD INTELLIGENT CHIP TECHNOLOGY
a. Introduction
b. Installation
c. Manufacturing Information
d. Column Use Information
VI.
TIPS AND TRICKS
VII.
CAUTIONARY NOTE
AX Columns
18
I. INTRODUCTION
Thank you for choosing a Waters™ Atlantis™ Premier
BEH C
AX Column. The Atlantis Premier BEH C
18
Column packing material is manufactured in a cGMP,
ISO 9001 certified plant using ultra-pure reagents. Each
batch is tested chromatographically and the results are
held to narrow specification ranges to assure excellent,
reproducible performance. Every column is individually
tested, and a Performance Chromatogram and Certificate
of Batch Analysis are provided in the column box or on the
eCord™ Intelligent Chip.
The Atlantis Premier BEH C
18
or without a VanGuard™ Fully Integrated Technology [FIT]
Cartridge. To address the desire to extend the operating
lifetimes of analytical columns, the VanGuard FIT Cartridge
is designed to prevent the non-desired introduction of sample
matrix or particulates onto the column without degrading
the separation. The VanGuard FIT Cartridge can be easily
replaced to restore separation performance and extend the
analytical column's lifetime.
The Atlantis Premier BEH C
18
High Performance Surfaces, which is an innovative technology
designed to increase analyte recovery, sensitivity, and
reproducibility by minimizing analyte/surface interactions
that can lead to sample losses.
AX
18
AX Column is offered with
AX Column utilizes MaxPeak™
1
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Related Manuals for Waters Atlantis Premier BEH C18 AX Columns
Summary of Contents for Waters Atlantis Premier BEH C18 AX Columns
- Page 1 [ CARE AND USE MANUAL ] Atlantis Premier BEH C AX Columns CONTENTS I. INTRODUCTION Thank you for choosing a Waters™ Atlantis™ Premier INTRODUCTION BEH C AX Column. The Atlantis Premier BEH C GETTING STARTED Column packing material is manufactured in a cGMP, a.
- Page 2 Intelligent Chip, the Certificate of Analysis and constant backpressure is achieved. Performance Test Chromatogram are available on request at Note: These columns may require longer initial equilibration times www.waters.com/coa. than conventional reversed-phase columns. a. eCord installation Table 1. Empty Column Volumes in mL...
- Page 3 For more information, visit www.waters.com/sampleprep on the Waters “Performance Test Chromatogram.” This is normal. The Waters isocratic column testing systems have 2. It is preferable to prepare the sample in the operating mobile been modified to achieve extremely low system volumes.
- Page 4 [ CARE AND USE MANUAL ] Table 2. Buffer Recommendations for Use with Atlantis Premier BEH C AX Columns Buffer Used for Additive/Buffer pK a Volatility Comments range Mass Spec — Volatile Ion pair additive, can suppress MS signal. Maximum buffering obtained when Acetic acid 4.76 —...
- Page 5 20-column volumes of solvent. Increasing column temperature increases cleaning efficiency. If the column performance is poor after regenerating and cleaning, call your local Waters office for additional support. b. Column storage Do not store columns in buffered eluents. If the mobile phase Figure 1.
- Page 6 Column use information eCord Intelligent Chip Figure 2. Waters eCord Intelligent Chip. At the time of manufacture, tracking and quality control information is downloaded to the eCord. Storing this information on the chip eliminates the need for a paper Certificate of Analysis.
- Page 7 [ CARE AND USE MANUAL ] VI. START UP: TIPS AND TRICKS FOR SUCCESS 1. Thiourea Atlantis Premier BEH C AX Columns are reversed-phase (RP) 2. 5-Fluorouracil 3. Nicotinamide 4. Procainamide columns. They differ from other C bonded, reversed-phase 5. Resorcinol 6.Adenosine 5'-monophosphate columns by utilizing an additional positive charge on the surface of the stationary phase.
- Page 8 Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. Waters, Atlantis, eCord, ACQUITY, UPLC, ACQUITY Arc, MaxPeak , Oasis, and Sep-Pak are trademarks T: 1 508 478 2000 of Waters Corporation. All other trademarks are the property of their respective owners.
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