Transmitted Light Brightfield Observation Procedures - Olympus BX53M Instructions Manual

3-15 Transmitted light brightfield observation procedures

1
2
Select the transmitted light illumination
3
Select the brightfield (BF) observation.
4
5
Select the light path. (Trinocular tube only)
6 Place a sample on the stage.
7 Engage the 10X objective in the light path.
8
Bring the sample into focus.
12
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
13 Adjust the field diaphragm.
46
Turn ON the main switch.
Adjust the brightness.
9
Adjust the interpupillary distance.
10
11 Adjust the optical axis.
14 Insert the required filters.
15
Start observation.
Adjust the diopter.
Adjust the brightness.
Main switch
Transmitted/reflected light switch
Observation method selector knob of the
reflected light illuminator
Brightness control knob of the microscope
frame
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Binocular tube
Diopter adjustment ring
Condenser height adjustment knob (page 48)
Condenser centering knob
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Field diaphragm ring of the microscope
frame
Filter mount of the microscope frame (page 50)
Brightness control knob of the microscope
frame
(page 16)
(page 16)
(page 17)
(page 26)
(page 19)
(page 19)
(page 19)
(page 23)
(page 24)
(page 32)
(page 32)
(page 48)
(page 23)
(page 24)
(page 48)
(page 26)