Adjusting The Prism - Olympus BX53M Instructions Manual

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Adjusting the prism

Turn the prism movement knob of the DIC slider to select the
interference color with the highest contrast according to the sample.
The interference color of the background changes
U-DICR :
continuously from the gray sensitive color to the
U-DICRHC :
magenta sensitive color (from -100 to 600 nm).
The interference color of the background changes
U-DICRH :
continuously from -100 to 100 nm.
· If you select the gray color for the background color, you can observe
the three dimensional image with high contrast in the gray sensitive
color with the highest sensitivity.
· If you select the magenta sensitive color for the background color,
even a small phase difference can be viewed by a variation of
the color.
If you want to select the magenta sensitive color for the
TIP
background color when using U-DICRH, engage the polarizer
U-POTP3 in the light path. For details, see "4-9 Setting the
analyzer and the polarizer of the reflected light illumination"
(page 60).
The detection sensitivity during DIC observation is very
NOTE
high. Be careful, particularly, for dirt or dust on the sample
surface.
The detection sensitivity is direction-oriented. If you turn the
TIP
sample using the rotational stage, the contrast could be
higher.
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