Transmitted Light Brightfield Observation Procedure - Olympus IX73 Instructions Manual

3
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
} The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic
observation method of this microscope. The operating procedures for phase contrast observation and DIC observa-
tion will be described separately in Chapter 5, "OTHER OBSERVATION METHODS" on page 33.
For the fluorescence observation, refer to the separate instruction manual entitled " Manual / Motorized Reflected
Fluorescence System".
Set the main switch to " I" (ON).
Select the (observation) light path with the
light path selector lever.
Place the specimen on the stage.
Engage a 10X objective in the light path.
Bring the specimen into focus.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the aperture iris and field iris diaphragms.
Start observation.
} When you turn OFF the power, turn OFF the main switch of IX3-CBM first and then turn OFF the main switch of the
other modules.7
12
} After turning ON the main switch of the
module to be used, turn ON the main
switch of IX3-CBM at last.
Disengage the DIC slider and
analyzer from the light path.
Engage the filters to be used in the
light path.
Adjust the brightness.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Engage the required filters.
Adjust the brightness.
(Controls Used)
@
Main switch: " I" (ON)
2
Lamp ON-OFF button: ON
3
Light intensity control knob
4
Filter pocket
5
Light path selector lever
6
X-axis and Y-axis knobs
7
Revolving nosepiece
8
Coarse/fine adjustment knobs
3
Light intensity control knob
9
Binocular observation tube
a
Diopter adjustment ring
b
Condenser height adjustment knob
c
Condenser centering knobs
d
Field iris diaphragm lever
7
Revolving nosepiece
8
Coarse/fine adjustment knobs
e
Aperture iris diaphragm lever
d
Field iris diaphragm lever
7
Filter pocket
3
Light intensity control knob
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