Transmitted Light Brightfield Observation Procedure - Olympus IX81 Instructions Manual

TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for phase contrast observation and DIC observation will be
described separately in Chapter 5, "OTHER OBSERVATION METHODS" on page 28.
For the fluorescence observation, refer to the separate instruction manual entitled "Reflected Fluorescence System".
Set the main switch to " I " (ON).
Engage a 10X objective in the light path.
Select the brightfield observation by pressing
the BF button* and select the
light path.
Place the specimen on the stage.
Bring the specimen in focus.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the aperture iris and field iris diaphragms.
Start observation.
* Usable when the DIA OBS setting is made in the IX2-BSW (Ver. 01.03 or later) software.
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Disengage the DIC slider and
analyzer from the light path.
Disengage the filter other than the
frost filter from the light path.
(observation)
Adjust the brightness.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Engage the required filters.
Adjust the brightness.
(Controls Used)
1Main switch: " I " (ON)
2Transmitted light ON-OFF button: ON
3Filter pocket
7Objective switching button
4BF observation button*
5Light path selector button
6X-axis and Y-axis knobs
8Focusing knob (dial)
Focus adjustment knob or
focusing button
9Light intensity control buttons
aBinocular tube
bDiopter adjustment ring
cCondenser height adjustment knob
dCondenser centering knobs
eField iris diaphragm lever
7Objective switching button
8Focusing knob (dial)
Focus adjustment knob
fAperture iris diaphragm lever
eField iris diaphragm lever
3Filter pocket
9Light intensity control buttons
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