Reflected Light Simple Polarization Observation Procedures - Olympus BX53M Instructions Manual

4-2 Reflected light simple polarization observation procedures

1 Turn ON the main switch.
2 Select the reflected light illumination
3
Select the differential interference contrast
/ polarization (DIC/POL) observation.
4
Set the analyzer and the polarizer.
5 Select the light path. (Trinocular tube only)
6 Place a sample on the stage.
7 Engage the 10X objective in the light path.
8 Bring the sample into focus.
12
Adjust the aperture diaphragm and the field diaphragm.
13 Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Start observation.
9 Adjust the brightness.
10
Adjust the interpupillary distance.
11
Adjust the diopter.
14 Adjust the brightness.
Main switch
Transmitted/reflected light switch
Observation method selector knob of the
reflected light illuminator
Analyzer insertion slot / polarizer insertion slot
of the reflected light illuminator
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
Binocular tube
Diopter adjustment ring
Aperture diaphragm knob of the reflected
light illuminator
Field diaphragm knob of the reflected light
illuminator
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
BX53M
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(page 60)
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(page 23)
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