Transmitted Light Brightfield Observation Procedure - Olympus BX45 Instructions Manual

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION
PROCEDURE
}As the phase contrast, darkfield and simplified polarized light observations using transmitted light requires preparation using
optical devices such as an analyzer or polarizer, they will additionally be detailed in section 4, "OBSERVATION".
Set the main switch to " I " (ON) and adjust the
brightness.
Select the BF (brightfield) light path by turning
the turret.
Select the light path (trinocular tube).
Place the specimen on the stage.
Engage the 10X objective in the light path.
Then engage the top lens in the light path.
Bring the specimen in focus.
Adjust the aperture iris and field iris diaphragms.
Engage the desired objective in the light path
and bring the specimen in focus.
Start observation.
6
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Insert the required filters.
Adjust the brightness.
(Controls Used)
@Main switch
²Brightness adjustment knob
³Turret
|Light path selector knob
ƒSlide holder
...X-/Y-axis knobs
†Revolving nosepiece
‡Top lens swing-out lever
ŠCoarse/fine adjustment knobs
‰Binocular tube
‹Diopter adjustment ring
ŒCondenser height adjustment
knob
™Condenser centering screws
šAperture iris diaphragm ring
›Field iris diaphragm ring
†Revolving nosepiece
‡Top lens swing-out lever
œFilters
²Brightness adjustment knob
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