Transmitted Light Brightfield Observation Procedure - Olympus IX71 Instructions Manual

TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic
observation method of this microscope. The operating procedures for phase contrast observation and DIC observation
will be described separately in Chapter 5, "OTHER OBSERVATION METHODS" on page 32.
For the fluorescence observation, refer to the separate instruction manual entitled "Manual/Motorized Reflected Fluores-
cence System".
Set the main switch to " I " (ON).
Select the (observation) light path with the
light path selector lever.
Place the specimen on the stage.
Engage a 10X objective in the light path.
Bring the specimen into focus.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the aperture iris and field iris diaphragms.
Start observation.
* This operation is not required when the IX2-ILL30 illumination column is used.
12
Disengage the DIC slider and
analyzer from the light path.
Disengage the filter other than the
frost filter from the light path.
Adjust the brightness.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Engage the required filters.
Adjust the brightness.
(Controls Used)
@Main switch: " I " (ON)
²Lamp ON-OFF button: ON
³Light intensity control knob
|Filter pocket
ƒLight path selector lever
...X-axis and Y-axis knobs
†Revolving nosepiece
‡Coarse/fine adjustment knobs
³Light intensity control knob
ŠBinocular observation tube
‰Diopter adjustment ring
‹Condenser height adjustment knob* (P. 27/
28)
ŒCondenser centering knobs*
™Field iris diaphragm lever*
†Revolving nosepiece
‡Coarse/fine adjustment knobs
šAperture iris diaphragm lever
™Field iris diaphragm lever*
|Filter pocket
³Light intensity control knob
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