Olympus BX53M-P Instructions Manual
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Olympus BX53M-P Instructions Manual

Olympus BX53M-P Instructions Manual

Polarizing microscope
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INSTRUCTIONS
BX53/BX53M-P
POLARIZING MICROSCOPE
Optical Microscope and Accessory
This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and
to familiarize yourself fully with the use of your microscope, we recommend that you read this manual
together with the instruction manual for the BX53/BX53M microscope thoroughly before use. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 9 8 8

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  • Page 1 POLARIZING MICROSCOPE Optical Microscope and Accessory This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and to familiarize yourself fully with the use of your microscope, we recommend that you read this manual together with the instruction manual for the BX53/BX53M microscope thoroughly before use. Retain this instruction manual in an easily accessible place near the work desk for future reference.

  • Page 3: Table Of Contents

    BX53/BX53M-P CONTENTS IMPORTANT ......................................1 NOMENCLATURE ..................................2 ASSEMBLY ....................................... 2-1 Assembly Diagram ......................................2-2 Detailed Assembly Procedure ................................3 CONTROLS ....................................4 USING THE CONTROLS ..............................4-1 Stage ............................................4-2 Intermediate Polarizing Attachment .............................. 5 POLARIZED LIGHT OBSERVATION ..........................

  • Page 4: Important

    U-CA or U-ECA magnification changer, etc.). When using an additional intermediate attachment, please make sure with your Olympus representative or the latest brochure. 3. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.

  • Page 5: Nomenclature

    BX53/BX53M-P NOMENCLATURE    Eyepiece Observation tube BX53/BX53M Microscope frame Polarizing intermediate attachment Centerable revolving nosepiece Objective (Strain-free objective series) Polarizing rotatable stage Strain-free condenser *Components for polarized light observation...

  • Page 6: Assembly

    ASSEMBLY 2-1 Assembly Diagram The diagram below shows how to assemble the various components. The numbers indicate the order of assembly. } For details on the BX53/BX53M microscope frame, consult the BX53/BX53M manual. When assembling the components, make sure that all parts are free of dust and dirt, and avoid scratching any CAUTION parts or touching glass surfaces.

  • Page 7: Detailed Assembly Procedure

    BX53/BX53M-P 2-2 Detailed Assembly Procedure Attaching the Stage (U-SRP) (Fig. 1) 1. Turn the coarse adjustment knob @ to lower the substage assembly ² to its lower limit. 2. Loosen the centering knobs ³. 3. Position the stage with the vernier scale | in front, and carefully lower the stage onto the round dovetail on the stage bracket ²...
  • Page 8 Mounting the Condenser (U-POC-2) (Fig. 3) 1. Turn the coarse adjustment knob @ to raise the stage to its upper limit. 2. Turn the condenser height adjustment knob ² to lower the condenser holder to its lower limit. 3. Loosen the condenser clamping screw ³. 4.
  • Page 9 BX53/BX53M-P Mounting the Objectives (Fig. 4) Insert the 10X or 20X objective into the primary hole (position where black rubber plugs are inserted into the centering holes on the nosepiece). Mount the other objectives in such a manner, that the magnification in-...
  • Page 10 Mounting the Intermediate Attachments (Fig. 6) 1. Using the Allen screwdriver, loosen the observation tube clamping screw @ on the microscope frame. 2. Insert the circular dovetail mount at the bottom of the intermediate attachments into the opening on the microscope frame and clamp by tightening the clamping screw @.
  • Page 11 BX53/BX53M-P Mounting the Observation Tube (Fig. 7) 1. Using the Allen screwdriver, fully loosen the observation tube clamping screw @ on the intermediate attachment. 2. Insert the circular dovetail mount at the bottom of the observation tube into the opening on the intermediate attachment, placing the observation tube to point the binocular eyepieces towards the front.
  • Page 12 Mounting Rotatable Analyzer (U-AN360P) (Fig. 9) 1. Place a desired ND filter (30 mm diam.) in the empty hole @ as required. 2. Insert the rotatable analyzer (U-AN360P) ² as far as the click-stop position. Then screw in the stopper knob ³. (Fig. 10) When using the fixed analyzer U-ANT instead of the rotatable analyzer U-AN360P, place the fixed analyzer in the test plate adapter U-TAD.

  • Page 13: Controls

    BX53/BX53M-P CONTROLS The illustration shows the U-CPA set. Other components are introduced on the following pages. Bertrand lens focusing knob Bertrand lens knob Analyzer Slider clamping knob Mechanical stage Slot for compensators Vernier scale Stage rotation clamping knob Aperture iris...
  • Page 14 Intermediate Attachment for Rotatable Analyzer (U-AN360P) orthoscopic observation (U-OPA) Empty hole (ND filter insertion possible) Analyzer slot Clamping knob Stopper knob Rotation dial Alignment polarizer Test Plate Adapter (U-TAD) Quarter Wave Plate (U-TP137) Sensitive Tint Plate (U-TP530) Test plate clamping knob Hole for the fixed analyzer (U-ANT) Slot for compensator plate...

  • Page 15: Using The Controls

    BX53/BX53M-P USING THE CONTROLS 4-1 Stage Specimen Placement When Using the Stage Clips (Fig. 11) Position the specimen in the center, and clamp the specimen with the stage clips. When Using the Mechanical Stage (U-FMP) (Fig. 12) Open the spring-loaded finger @ and place the specimen on the stage.
  • Page 16 Stage Rotation (Fig. 13) When the stage rotation clamping knob @ is loosened, the stage can be rotated horizontally through 360°. Fig. 13 Using 45° Click Stop Lever (Fig. 14) When the 45° click stop lever @, located at the right side of the stage, is moved toward the observer, and the stage is moved from this position to the first click-stop position, the specimen is moved 45°...
  • Page 17 BX53/BX53M-P Stage Height Adjustment (Figs. 15 & 16) } By lowering the position of the substage, the microscope will accommodate specimens with a maximum height of 35 mm. This is useful when observing metallurgical specimens and other thick objects. 1. Lower the stage to its lower limit, then remove the stage from the microscope.

  • Page 18: Intermediate Polarizing Attachment

    4-2 Intermediate Polarizing Attachment Using the Bertrand Lens (U-CPA only) (Fig. 17) By manipulating the Bertrand lens knob @ on the front, the Bertrand lens is inserted into or removed from the light path. At the pushed-in ({) position, the lens is engaged. At the pulled-out ( ) position, the lens is ¦...

  • Page 19: Polarized Light Observation

    BX53/BX53M-P POLARIZED LIGHT OBSERVATION 5-1 Adjustments Before Observation In polarized light microscopy ultimate performance is not obtainable unless optical adjustments are made correctly. Always performed the following adjustments before observation. Remove the quarter wave plate and sensitive tint plate from the light path.
  • Page 20 4. Slightly loosen the intermediate adapter clamping screw ƒ. (Fig. 20) 5. While observing the field of view, rotate the intermediate attachment for orthoscopic observation (U-OPA) | until complete extinction is obtained. At this point, tighten the intermediate attachment clamping screw ƒ. (Fig.
  • Page 21 BX53/BX53M-P (5) Clamp the stage with the stage rotation clamping knob. 10. This concludes centering of the optical axis for the 10X objective, which will act as the reference objective. Now, center the other objectives with the centerable nosepiece by engaging the objectives into the light path one after another.
  • Page 22 Note: During conoscopic observation, the center of the conoscopic image @ and the intersection of the cross lines ² may not coincide. However, employment of the universal infinity optical design ensures Cross lines ² that this disagreement will have no measurable effect during actual observation.
  • Page 23 BX53/BX53M-P 6. To brighten and facilitate observation of the conoscopic image during optical axis adjustment, rotate the analyzer slightly away from the position of total extinction. (Fig. 26) Conoscopic image Fig. 26 7. Insert the two provided centering wrenches ² into the centering holes for the primary hole with the 20X or 10X objective on the nosepiece.
  • Page 24 10. Centering the rotatable stage (Figs. 28 & 29) (1) Place the specimen. (2) Focus on the specimen and look for an easily recognizable detail ³ in the field. Move this detail in the center of the eyepiece cross lines. (3) When the stage is rotated, the detail moves in circle |.

  • Page 25: Adjusting For Extinction

    BX53/BX53M-P Adjusting for Extinction (Fig. 30) Remove specimen, test plate, compensator, etc. from the light CAUTION path. 1. Swing out the top lens of the condenser, and engage the 10X objective. 2. Insert the rotatable analyzer into the light path and set vibration direction scale at the 0°...

  • Page 26: Adjusting The Eyepiece Cross Lines

    Adjusting the Eyepiece Cross Lines (Fig. 31) } To align the eyepiece cross lines and the vibration direction, the orientation plate provided with the U-CPA or U-OPA is required. Orientation plate (U-PJ: Single module name) FIg. 31 Remove the test plate adapter and the compensator from the CAUTION light path.
  • Page 27 BX53/BX53M-P 3. Coincide the orientation plate’s center portion with the intersection of the eyepiece cross lines. Engage the analyzer into the light path (in case of U-AN360P, set the analyzer to position 0°) to obtain crossed filters position (extinction). 4. While observing, rotate the stage to locate the position where the orientation plate is darkest. At this point, clamp the stage.

  • Page 28: Orthoscopic Observation

    5-2 Orthoscopic Observation } In principle, polarized light enters the light path, parallel to the optical axis, to enable observation of the optical characteristics of the specimen. Therefore, swing out the top lens of the condenser. Use 4X to 100X objectives. 1.

  • Page 29: Conoscopic Observation

    BX53/BX53M-P 5-3 Conoscopic Observation } Use 20X to 100X objectives. 1. Engage the analyzer and adjust for extinction position. 2. Swing the condenser top lens into the light path. 3. When using the intermediate attachment conoscopic and orthoscopic observation U-CPA, push in the Bertrand lens knob to engage the Bertrand lens into the light path.

  • Page 30: Specifications

    SPECIFICATIONS Specifications Item Intermediate attachment conoscopic and Intermediate attachment for orthoscopic observation (U-CPA) orthoscopic observation (U-OPA) 1. Polarizing Field No. intermediate  Bertrand lens Focusable attachment  Bertrand aperture stop Fixed diaphragm (U-CPA and U-OPA) Bertrand lens knob position Pushed in position: ...
  • Page 31 BX53/BX53M-P Specifications Item Intermediate attachment conoscopic and Intermediate attachment for orthoscopic observation (U-CPA) orthoscopic observation (U-OPA) 5. Condenser Aplanatic/achromatic condenser, top lens swing-out type (U-POC-2) Polarizer 360° rotatable 0° position adjustable N.A. 0.9 (top lens swung in) Applicable objectives: 4X to 100X (4X objective is used with the top lens swung out) Operating environment ·...

  • Page 32: Optical Characteristics

    Mechanical tube length Field number (FN) NOTE Refer to the latest catalogue or consult your local Olympus representative for the updated information on the eyepieces Cover glass thickness Color band and objectives that can be combined with this microscope.

  • Page 33: Troubleshooting Guide

    Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Trouble...
  • Page 34 MEMO...
  • Page 36 Please contact our "Service Center" through the following website for any inquiries or issues related to this product. EVIDENT CORPORATION 6666 Inatomi, Tatsuno-machi, Kamiina-gun, Nagano 399-0495, Japan (Life science solutions) (Life science solutions) Service Center Our Website https://www.olympus-lifescience.com/support/ https://www.olympus-lifescience.com service/ (Industrial solutions) (Industrial solutions) Service Center Our Website https://www.olympus-ims.com/service-and- https://www.olympus-ims.com...

This manual is also suitable for:

Bx53

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