Adjusting During Observation; Centering Of The Aperture Diaphragm (As) - Olympus BX53M Instructions Manual

70%
30%
Aperture diaphragm
image
4
5
BX3M-RLAS-S, BX3M-URAS-S
4
5
BX3M-RLA-S
2

Adjusting during observation

Reflected light brightfield observation
When using BX3M-RLA-S, narrow down the aperture diaphragm to
between 70 and 80% of the numerical aperture of the objective to
perform the excellent observation.
Reflected light darkfield observation /
Reflected light fluorescence observation
Open the aperture diaphragm during the observation.
3

Centering of the aperture diaphragm (AS)

When centering the aperture diaphragm, be careful not to
NOTE
touch the X-axis/Y-axis knob of the stage with your arm.
Use the Allen screwdriver provided with the microscope to
TIP
perform the centering.
1
Select the brightfield observation (BF). For procedures to select the
observation method, see "3-4 Selecting the observation method"
(page 17).
2
Turn the nosepiece to engage the 10X objective in the light path.
3
Attach the total reflection mirror to the stage and bring the sample
into focus approximately.
4
Pull out the eyepiece. While looking into the eyepiece sleeve, move
the aperture diaphragm knob (aperture diaphragm lever) to adjust the
diameter of the aperture diaphragm image to approximately 70% of
the field of view.
5
If the aperture diaphragm image is displaced from the center of the
field of view, insert the Allen screwdriver to the AS centering screws
(2 pcs.) of the reflected light illuminator and turn each to adjust the
aperture diaphragm image to come to the center of the field of view.
BX53M
39