Molecular Devices SpectraMax Paradigm User Manual page 98

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
About Fluorescence Intensity
In fluorescence intensity read mode, the source light is directed through a tunable excitation
filter and then focused by an objective lens from above or below the microplate. The filter
passes only the specified wavelength band necessary to excite samples. The objective lens
collects the resulting fluorescence and directs it through a tunable emission filter to separate
background light from the specific wavelengths generated by samples. This signal is detected
by a photo multiplier tube.
The Tunable Wavelength (TUNE) Detection Cartridge contains filter sets for measuring
fluorescence intensity of most fluorophores that are compatible with the available
wavelength ranges. The uses high-power LEDs and tunable filters together with the photon
counting detection of the instrument, resulting in excellent detection limits and linear
dynamic range at short read times. See
Using a Spectrum read, you can define a fixed excitation wavelength and scan the emission
wavelengths, or define a fixed emission wavelength and scan the excitation wavelengths. The
spectral optimization helps to get the maximum signal-to-background ratio from a
fluorescent or fluorescently labeled analyte. For more information on using the spectral
optimization feature, see the SoftMax Pro Software application help or user guide.
For more information about fluorescence intensity, see
on page
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28.
Measurement Specifications on page
Fluorescence Intensity Read Mode
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