Molecular Devices SpectraMax Paradigm User Manual page 108

SpectraMax Paradigm Multi-Mode Detection Platform User Guide
About AlphaScreen
ALPHA stands for Amplified Luminescent Proximity Homogeneous Assay. AlphaScreen is a
bead-based chemistry used to study molecular interactions between moieties A and B, for
example. When a biological interaction between A and B moves beads—coated with A and B,
respectively—together, a cascade of chemical reactions produce a greatly amplified signal.
An AlphaScreen measurement includes a light pulse, by turning on the laser diode for a
specified time, turning off the laser diode, followed by the measurement of the AlphaScreen
signal, as specified in the protocol parameters.
Table 5-7: AlphaScreen HTS Timing Parameters
Parameter
Excitation time
Integration time
For more information about AlphaScreen, see
Microplate Recommendations
The following information is specific to this detection cartridge. For general microplate
selection and handling guidelines, see
Table 5-8: Microplate Selection Guidelines for the AlphaScreen Detection Cartridge
Read Mode
AlphaScreen
(ALPHA)
108
Value Comment
40 ms The period when the sample is exposed to the laser.
80 ms The period when the signal is read from the sample.
Microplate Other Considerations
Type
Solid white When an application specifies a surface treatment, use only
microplates with the correct treatment.
White plates are recommended for reading AlphaScreen (such as,
Optiplate-384 white opaque, Perkin Elmer #6007290). Black plates
absorb light and lead to reduced signals.
AlphaScreen Read Mode on page
Selecting Suitable Microplate Types on page
53.
91.
5014038 E