Molecular Devices SpectraMax Paradigm User Manual page 129

About Luminescence
Luminescence is the emission of light by processes that derive energy from essentially non-
thermal changes, the motion of subatomic particles, or the excitation of an atomic system by
radiation. Luminescence detection relies on the production of light from a chemical reaction
in a sample.
Each Glow Luminescence (LUM) Detection Cartridge contains the components for measuring
the light intensity from luminescence. Since the light is emitted as a result of a chemical
reaction, no excitation light and no excitation filters are required to measure luminescence.
The luminescence can be measured with no emission filters, or an emission wavelength
selected from within the specified range. See
Note: This detection cartridge is not suitable for measurement of flash luminescence
reactions, which require injectors.
For more information about luminescence, see
Microplate Recommendations
The following information is specific to this detection cartridge. For general microplate
selection and handling guidelines, see
Table 5-22: Microplate Selection Guidelines for the Glow Luminescence (LUM) Detection
Cartridges
Read Mode
Luminescence
(LUM)
Note: White microplates provide significantly higher signal than black microplates,
and are recommended if high sensitivity is required. White microplates can, however,
exhibit some detectable phosphorescence which increases background after being
exposed to light (in particular under neon lights). For maximum sensitivity, Molecular
Devices recommends to prepare microplates under reduced ambient light conditions,
and to adapt the microplates to dark for 10 to 30 minutes before measurement.
5014038 E
Selecting Suitable Microplate Types on page
Microplate Other Considerations
Type
Solid white When an application specifies a surface treatment, use only
microplates with the correct treatment.
Molecular Devices recommends using an unlidded plate for
luminescence measurements.
Chapter 5: Available Detection Cartridges
Measurement Specifications on page
Luminescence Read Mode on page
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