Molecular Devices SpectraMax Paradigm User Manual page 43

Suggested timing parameters for a dual-label Europium-Samarium measurement are listed in
the following table.
Table 2-3: Time-resolved fluorescence timing parameters for dual-label Europium-
Samarium
Parameter
Pulse length
Measurement delay (first
window)
Integration time (first window)
Measurement delay (second
window)
Integration time (second
window)
5014038 E
Value Comment
0.100 ms The time interval for flash monitoring
This is the suggested value for the TRF detection
cartridge.
0.010 ms The delay to ensure the excitation pulse is no longer
detectable
This is the suggested value for the TRF detection
cartridge.
0.100 ms The period for accumulating the Samarium signal
This is the suggested value for the TRF detection
cartridge.
0.140 ms The read out of the Samarium signal
This is the suggested value for the TRF detection
cartridge.
0.750 ms The period for accumulating the Europium signal
This is the suggested value for the TRF detection
cartridge.
Chapter 2: Read Modes and Read Types
43