Molecular Devices SpectraMax Paradigm User Manual page 21

Well Scan Read Type
A Well Scan read can take readings at more than one location within a well. A Well Scan read
takes one or more readings of a single well of a microplate on an evenly spaced grid inside of
each well at single or multiple wavelengths.
Some applications involve the detection of whole cells in large-area tissue culture plates. Well
Scan reads can be used with such microplates to permit maximum surface area detection in
whole-cell protocols. Since many cell lines tend to grow as clumps or in the corners of
microplate wells, you can choose from several patterns and define the number of points to
be scanned to work best with your particular application.
The following scanning patterns are available:
A horizontal line
A vertical line
A cross pattern
A fill pattern
The fill pattern can be either round or square to match the shape of the well. The image in
the Well Scan settings shows the shape of the well as defined for the selected microplate.
You can set the density of the well scan to determine the number of points to read in a line
pattern or the maximum number of horizontal and vertical points included in a cross or fill
pattern.
Depending on the read mode selected, the values are reported as optical density (OD),
%Transmittance (%T), relative fluorescence units (RFU), or relative luminescence units (RLU).
Spectrum Read Type
Depending on the read mode selected, a Spectrum read measures optical density (OD),
%Transmittance (%T), relative fluorescence units (RFU), or relative luminescence units (RLU)
across a spectrum of wavelengths.
Spectrum reads are available only for specific detection cartridges. See
Cartridges on page
5014038 E
93.
Chapter 2: Read Modes and Read Types
Available Detection
21